Recombinant
RabMAb

Anti-Transferrin Receptor antibody [EPR4013] (ab109259)

Overview

  • Product name
    Anti-Transferrin Receptor antibody [EPR4013]
    See all Transferrin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR4013] to Transferrin Receptor
  • Tested applications
    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human Transferrin Receptor.

  • Positive control
    • Human placenta, MCF7, and JAR lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab109259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in disease
      Immunodeficiency 46
    • Sequence similarities
      Belongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      modifications
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localization
      Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • IMD46 antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Images

    • All lanes : Anti-Transferrin Receptor antibody [EPR4013] (ab109259) at 1/10000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : MCF7 lysate
      Lane 3 : JAR lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    References

    ab109259 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109259.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up