Anti-Transferrin Receptor antibody [EPR4013] (ab109259)

Overview

  • Product nameAnti-Transferrin Receptor antibody [EPR4013]
    See all Transferrin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR4013] to Transferrin Receptor
  • Tested applicationsSuitable for: WB, IPmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human Transferrin Receptor.

  • Positive control
    • Human placenta, MCF7, and JAR lysates.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109259 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 84 kDa).
IP 1/10 - 1/100.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
      (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
    • Involvement in diseaseImmunodeficiency 46
    • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
      Contains 1 PA (protease associated) domain.
    • Post-translational
      modifications
      N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
      Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
      Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
    • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links
    • Alternative names
      • CD 71 antibody
      • CD71 antibody
      • CD71 antigen antibody
      • OTTHUMP00000208523 antibody
      • OTTHUMP00000208524 antibody
      • OTTHUMP00000208525 antibody
      • p90 antibody
      • sTfR antibody
      • T9 antibody
      • TFR 1 antibody
      • TfR antibody
      • TfR1 antibody
      • TFR1_HUMAN antibody
      • TFRC antibody
      • TR antibody
      • Transferrin receptor (p90 CD71) antibody
      • Transferrin receptor protein 1, serum form antibody
      • Trfr antibody
      see all

    Anti-Transferrin Receptor antibody [EPR4013] images

    • All lanes : Anti-Transferrin Receptor antibody [EPR4013] (ab109259) at 1/10000 dilution

      Lane 1 : Human placenta lysate
      Lane 2 : MCF7 lysate
      Lane 3 : JAR lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 84 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)

    References for Anti-Transferrin Receptor antibody [EPR4013] (ab109259)

    ab109259 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109259.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"