Anti-Transferrin Receptor antibody [MEM-189] (ab1086)

Overview

  • Product nameAnti-Transferrin Receptor antibody [MEM-189]
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [MEM-189] to Transferrin Receptor
  • SpecificityThe antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor.
  • Tested applicationsSuitable for: IHC-FrFl, Flow Cyt, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Tissue/ cell preparation (Human). (KG1 acute myelogenous leukaemia cell line).

Properties

Applications

Our Abpromise guarantee covers the use of ab1086 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FrFl Use at an assay dependent concentration.
Flow Cyt Use a concentration of 2 µg/ml.
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Use under non reducing condition.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [MEM-189] images

  • ab1086 staining Transferrin Receptor in Human PC-3 prostate cancer cell line by Flow Cytometry. The sample was incubated with the primary antibody (0.3µg/ml in PBS + 2% FBS) for 1 hour at 4°C. An Alexa Fluor® 488-conjugated Goat anti-mouse IgG (1/500) was used as the secondary antibody.
    Gating Strategy: Cells gated for live and singlets.

    See Abreview

  • ab1086 at 1/100 dilution, staining Transferrin Receptor in rat mesenchymal stem cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed with 4% paraformaldehyde for 10 min at room temperature, washed with PBS (pH 7.4) and incubated with 10% serum plus background reducing components, for 30 min at room temperature. A Fluorescene conjugated anti-mouse IgG was used as secondry antibody and observed under an epi-fluorescence microscope (Nikon, Tokyo, Japan).

References for Anti-Transferrin Receptor antibody [MEM-189] (ab1086)

This product has been referenced in:
  • Leung SJ  et al. In vivo molecular mapping of the tumor microenvironment in an azoxymethane-treated mouse model of colon carcinogenesis. Lasers Surg Med 47:40-9 (2015). Read more (PubMed: 25487746) »
  • Opazo F  et al. Aptamers as potential tools for super-resolution microscopy. Nat Methods 9:938-9 (2012). Read more (PubMed: 23018995) »

See all 8 Publications for this product

Product Wall

Application Flow Cytometry
Fixation None
Permeabilization No
Sample Human Cell (PC-3 Prostrate cancer cell line)
Specification PC-3 Prostrate cancer cell line
Gating Strategy Cells gated for live and singlets
Preparation Cell harvesting/tissue preparation method: Cells detached with PBS-based cell dissociation buffer, collected by centrifugation and re-suspended in PBS + 2% FBS
Sample buffer: PBS + 2% FBS
Username

Abcam user community

Verified customer

Submitted Nov 05 2013

Thank you for contacting us.

According to our datasheet, this antibody only works under non-reducing conditions, I am afraid: it may be that the antibody cannot detect the reduced protein.Otherwise, it should workwith the use of your usual p...

Read More

Thank you for contacting us.

Unfortunately the epitope of ab1086 has not been mapped. We only know that this antibody recognise an extracellular part of Transferrin Receptor.

I am sorry for not being able to provide any useful info...

Read More

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has cau...

Read More

Thank you for contacting us.

Yes, the extra blocking step is always required to avoid the mouse back ground. We have recommendations that can be found by clicking the following link;

http://www.abcam.com/index.html?pageconfig=resou...

Read More

Thank you for your enquiry.

We have not tested these products (ab1086, ab84036, ab9179, ab8598) for cross reactivity with Trf2. However, based on immunogen sequence (ClustalW analysis, Score 23.0), we do not expect to see any cross reactivit...

Read More

Thank you for bringing that to my attention.
I did some additional research into CD71. CD71, also known as the transferrin receptor (TFR), is a type II membrane glycoprotein that exists as a disulfide-linked homodimer of two identical subunits. Ab...

Read More

Thank you for your response.

I am very sorry to say this but we cannot accept returned items that have been frozen. This is because we need to limit any freeze/thaw cycles that the product might experience. I am sorry about the inconvenience t...

Read More

I am sorry that you have been experiencing problems with one of our products and wish to thank you for bringing this to our attention. As this order was placed and delivered within the last seven day I may issue a return for you. Since this is ultimate...

Read More

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from ab1086.

I would like to reassure you that In the event that a product is not functioning in the applications and spec...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"