Anti-Transferrin Receptor antibody [MEM-189] (ab1086)
Key features and details
- Mouse monoclonal [MEM-189] to Transferrin Receptor
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Transferrin Receptor antibody [MEM-189]
See all Transferrin Receptor primary antibodies -
Description
Mouse monoclonal [MEM-189] to Transferrin Receptor -
Host species
Mouse -
Specificity
The antibody MEM-189 reacts with CD71 antigen (transferrin receptor), a 95 kDa type II homodimeric transmembrane glycoprotein expressed on activated B and lymphocytes, macrophages and erythroid precursors; it is lost on resting blood leukocytes. The antibody MEM-189 does not block binding of transferrin to the receptor. -
Tested applications
Suitable for: Flow Cytmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human Transferrin Receptor. KG1 human acute myelogenous leukaemia cell line
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Positive control
- Flow Cyt: K562 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 8.0
Preservative: 0.097% Sodium azide
Constituent: Tris buffer -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MEM-189 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1086 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt | (1) |
Use a concentration of 2 µg/ml.
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Notes |
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Flow Cyt
Use a concentration of 2 µg/ml. |
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 7037 Human
- Omim: 190010 Human
- SwissProt: P02786 Human
- Unigene: 529618 Human
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (31)
ab1086 has been referenced in 31 publications.
- Zhang S et al. Targeting ferroptosis with miR-144-3p to attenuate pancreatic β cells dysfunction via regulating USP22/SIRT1 in type 2 diabetes. Diabetol Metab Syndr 14:89 (2022). PubMed: 35761309
- Düsterhöft S et al. The iRhom homology domain is indispensable for ADAM17-mediated TNFα and EGF receptor ligand release. Cell Mol Life Sci 78:5015-5040 (2021). PubMed: 33950315
- Shan X et al. The Protective Effect of Cyanidin-3-Glucoside on Myocardial Ischemia-Reperfusion Injury through Ferroptosis. Oxid Med Cell Longev 2021:8880141 (2021). PubMed: 33628391
- Zeng X et al. Benefits of Iron Chelators in the Treatment of Parkinson's Disease. Neurochem Res 46:1239-1251 (2021). PubMed: 33646533
- Zhang Y et al. Salvia miltiorrhiza (SM) Injection Ameliorates Iron Overload-Associated Cardiac Dysfunction by Regulating the Expression of DMT1, TfR1, and FP1 in Rats. Evid Based Complement Alternat Med 2021:6864723 (2021). PubMed: 34135983