Anti-Transferrin Receptor antibody [MRC OX-26] (Biotin) (ab112215)

Overview

  • Product nameAnti-Transferrin Receptor antibody [MRC OX-26] (Biotin)
    See all Transferrin Receptor primary antibodies
  • Description
    Mouse monoclonal [MRC OX-26] to Transferrin Receptor (Biotin)
  • ConjugationBiotin
  • Specificityab112215 does not block binding of transferrin to CD71.
  • Tested applicationsSuitable for: IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
  • Immunogen

    Tissue, cells or virus corresponding to Rat Transferrin Receptor.

  • Positive control
    • Rat strain: Wistar (Bone Marrow, T Cell Blasts or Spleen cells)

Properties

Applications

Our Abpromise guarantee covers the use of ab112215 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 1µg for 106 cells. ab97679-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • IMD46 antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [MRC OX-26] (Biotin) images

  • Flow cytometry staining of CD71 in T Cell Blasts using ab112215 at 1 µg for 106 cells.

    Percentage of cells stained above control: 86.2%

References for Anti-Transferrin Receptor antibody [MRC OX-26] (Biotin) (ab112215)

ab112215 has not yet been referenced specifically in any publications.

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Regarding ab112215: The immunogen used to make clone MRC OX-26 was PHA activated lymph node cells of PVG rat. As far as we are aware, the epitope of this...

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Thank you for contacting Abcam. I have attached the protocol that was used to test ab112215 in flow cytometry. To our knowledge, ab112215 has not been tested in CHO cells. Therefore, I can offer a discount off a future purchase if you buy ab112215 now,...

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