FITC Anti-Transferrin Receptor antibody [OX-26] (ab33927)
Key features and details
- FITC Mouse monoclonal [OX-26] to Transferrin Receptor
- Suitable for: Flow Cyt
- Reacts with: Rat
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-Transferrin Receptor antibody [OX-26]
See all Transferrin Receptor primary antibodies -
Description
FITC Mouse monoclonal [OX-26] to Transferrin Receptor -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
Recognizes the rat transferrin receptor (CD71). It labels dividing cells of various types but also binds to a number of non-dividing normal tissues and endothelium of brain capillaries. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Rat -
Immunogen
Tissue, cells or virus corresponding to Rat Transferrin Receptor. PHA-activated rat lymphocytes.
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Positive control
- PHA-activated rat lymphocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
OX-26 -
Myeloma
NS1 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33927 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. Use 10ul of the suggested working dilution to label 10^6 cells in 100ul. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Function
Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
(Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus. -
Involvement in disease
Immunodeficiency 46 -
Sequence similarities
Belongs to the peptidase M28 family. M28B subfamily.
Contains 1 PA (protease associated) domain. -
Post-translational
modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation. -
Cellular localization
Secreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 64678 Rat
- SwissProt: Q99376 Rat
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Alternative names
- CD 71 antibody
- CD71 antibody
- CD71 antigen antibody
see all
Datasheets and documents
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Datasheet download
References (1)
ab33927 has been referenced in 1 publication.
- Rathnasamy G et al. Iron and Iron Regulatory Proteins in Amoeboid Microglial Cells Are Linked to Oligodendrocyte Death in Hypoxic Neonatal Rat Periventricular White Matter through Production of Proinflammatory Cytokines and Reactive Oxygen/Nitrogen Species. J Neurosci 31:17982-95 (2011). IHC (PFA fixed) ; Rat . PubMed: 22159112