The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. In vitro inhibition of cell proliferation.
Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240). (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
Involvement in diseaseImmunodeficiency 46
Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily. Contains 1 PA (protease associated) domain.
Post-translational modificationsN- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated. Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR). Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
Flow cytometry analysis staining Transferrin Receptor in pre-B cell line 18-81 using ab25622 at a dilution of 0.1 ug/106 cells. Rat anti-mouse CD71-PE was used as an isotype control.
References for Anti-Transferrin Receptor antibody [R17217] (Phycoerythrin) (ab25622)
This product has been referenced in:
Martín-Jaular L et al. Reticulocyte-prone malaria parasites predominantly invade CD71hi immature cells: implications for the development of an in vitro culture for Plasmodium vivax. Malar J12:434 (2013).
Read more (PubMed: 24289105) »
Kemp JD et al. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol138:2422-6 (1987).
Read more (PubMed: 2951439) »