Anti-Transferrin Receptor antibody [R17217] (Phycoerythrin) (ab25622)

Overview

  • Product nameAnti-Transferrin Receptor antibody [R17217] (Phycoerythrin)
    See all Transferrin Receptor primary antibodies
  • Description
    Rat monoclonal [R17217] to Transferrin Receptor (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Tested applicationsSuitable for: Inhibition Assay, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    The details of the immunogen for this antibody are not available.

Properties

Applications

Our Abpromise guarantee covers the use of ab25622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Inhibition Assay Use at an assay dependent concentration. In vitro inhibition of cell proliferation.
Flow Cyt Use 0.2µg for 106 cells. ab134674-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

Anti-Transferrin Receptor antibody [R17217] (Phycoerythrin) images

  • Flow cytometry analysis staining Transferrin Receptor in pre-B cell line 18-81 using ab25622 at a dilution of 0.1 ug/106 cells. Rat anti-mouse CD71-PE was used as an isotype control.

References for Anti-Transferrin Receptor antibody [R17217] (Phycoerythrin) (ab25622)

This product has been referenced in:
  • Martín-Jaular L  et al. Reticulocyte-prone malaria parasites predominantly invade CD71hi immature cells: implications for the development of an in vitro culture for Plasmodium vivax. Malar J 12:434 (2013). Flow Cyt ; Mouse . Read more (PubMed: 24289105) »
  • Kemp JD  et al. Role of the transferrin receptor in lymphocyte growth: a rat IgG monoclonal antibody against the murine transferrin receptor produces highly selective inhibition of T and B cell activation protocols. J Immunol 138:2422-6 (1987). Read more (PubMed: 2951439) »

See all 2 Publications for this product

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