Overview

  • Product nameAnti-Transferrin Receptor antibody [YTA 74.4]
    See all Transferrin Receptor primary antibodies
  • Description
    Rat monoclonal [YTA 74.4] to Transferrin Receptor
  • SpecificityThis antibody detects mouse CD71 antigen, the transferrin receptor (TFR). Expressed on all dividing cells and can distinguish resting and activated T-cells. Immunoprecipitates a disulphide linked homodimer of 90kD by SDS PAGE. The antibody inhibits cell proliferation and the mixed lymphocyte response in vitro. Blocks the binding of R17 217.1.3. and R17 208.2 anti-TFR monoclonal antibodies.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IP, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat
  • Immunogen

    Tissue/ cell preparation (Mouse). Mouse Concanavilin A activated spleen cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab22391 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration. PubMed: 20457837
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 1 x 106 cells.



ab18450-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IP Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

References for Anti-Transferrin Receptor antibody [YTA 74.4] (ab22391)

This product has been referenced in:
  • Mukai Y  et al. Induction of endoplasmic reticulum-endosome fusion for antigen cross-presentation induced by poly (?-glutamic acid) nanoparticles. J Immunol 187:6249-55 (2011). IHC (PFA fixed) ; Mouse . Read more (PubMed: 22095716) »
  • Makhro A  et al. Functional NMDA receptors in rat erythrocytes. Am J Physiol Cell Physiol 298:C1315-25 (2010). ICC/IF ; Rat . Read more (PubMed: 20457837) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"