Anti-Transferrin Receptor antibody [YTA 74.4] (FITC) (ab33996)

Overview

  • Product nameAnti-Transferrin Receptor antibody [YTA 74.4] (FITC)
    See all Transferrin Receptor primary antibodies
  • Description
    Rat monoclonal [YTA 74.4] to Transferrin Receptor (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Concanavilin A activated mouse spleen cells.

  • General notes


    ab33996 stains dividing cells, and can be used to distinguish resting from activated T cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab33996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells.

Use 10µl to label 106 cells in 100µl.





ab18446-Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • FunctionCellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system (By similarity). A second ligand, the heditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site. Positively regulates T and B cell proliferation through iron uptake (PubMed:26642240).
    (Microbial infection) Acts as a receptor for new-world arenaviruses: Guanarito, Junin and Machupo virus.
  • Involvement in diseaseImmunodeficiency 46
  • Sequence similaritiesBelongs to the peptidase M28 family. M28B subfamily.
    Contains 1 PA (protease associated) domain.
  • Post-translational
    modifications
    N- and O-glycosylated, phosphorylated and palmitoylated. The serum form is only glycosylated.
    Proteolytically cleaved on Arg-100 to produce the soluble serum form (sTfR).
    Palmitoylated on both Cys-62 and Cys-67. Cys-62 seems to be the major site of palmitoylation.
  • Cellular localizationSecreted and Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • Database links
  • Alternative names
    • CD 71 antibody
    • CD71 antibody
    • CD71 antigen antibody
    • OTTHUMP00000208523 antibody
    • OTTHUMP00000208524 antibody
    • OTTHUMP00000208525 antibody
    • p90 antibody
    • sTfR antibody
    • T9 antibody
    • TFR 1 antibody
    • TfR antibody
    • TfR1 antibody
    • TFR1_HUMAN antibody
    • TFRC antibody
    • TR antibody
    • Transferrin receptor (p90 CD71) antibody
    • Transferrin receptor protein 1, serum form antibody
    • Trfr antibody
    see all

References for Anti-Transferrin Receptor antibody [YTA 74.4] (FITC) (ab33996)

This product has been referenced in:
  • Fagoonee S  et al. Lack of plasma protein hemopexin dampens mercury-induced autoimmune response in mice. J Immunol 181:1937-47 (2008). Flow Cyt ; Mouse . Read more (PubMed: 18641331) »
  • Lesley J  et al. Expression of transferrin receptor on murine hematopoietic progenitors. Cell Immunol 83:14-25 (1984). Read more (PubMed: 6319033) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"