Overview

  • Product nameAnti-Transglutaminase 2 antibody
    See all Transglutaminase 2 primary antibodies
  • Description
    Goat polyclonal to Transglutaminase 2
  • Tested applicationsSuitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Guinea pig
  • Immunogen

    Full length native protein (purified) (Guinea pig).

  • Positive control
    • Mouse liver, testes, thymus, spleen and kidney tissue extracts.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab10445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IP
  • Application notesIP: Use 15 µl antibody per 500-1000 µg protein extract from mouse liver or spleen extract. Does not IP from mouse kidney extract.
    WB: 1/1000. Detects a band of approximately 78 kDa.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionCatalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
    • Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
    • Information by UniProt
    • Database links
    • Alternative names
      • ALPHA SUBUNIT antibody
      • C polypeptide antibody
      • EC 2.3.2.13 antibody
      • epididymis secretory protein Li 45 antibody
      • G alpha h antibody
      • G[a]h antibody
      • Gh CLASS G ALPHA h antibody
      • GNAH antibody
      • GNAH G PROTEIN antibody
      • H POLYPEPTIDE antibody
      • HEL-S-45 antibody
      • Protein glutamine gamma glutamyltransferase 2 antibody
      • Protein-glutamine gamma-glutamyltransferase 2 antibody
      • TG 2 antibody
      • TG(C) antibody
      • TG2 antibody
      • TGase C antibody
      • TGase H antibody
      • TGase-2 antibody
      • TgaseII antibody
      • TGC antibody
      • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
      • TGM2 antibody
      • TGM2_HUMAN antibody
      • Tissue transglutaminase antibody
      • Transglutaminase 2 antibody
      • Transglutaminase 2 C polypeptide antibody
      • Transglutaminase C antibody
      • Transglutaminase H antibody
      • Transglutaminase-2 antibody
      • tTG antibody
      • tTGas antibody
      see all

    Anti-Transglutaminase 2 antibody images

    • All lanes : Anti-Transglutaminase 2 antibody (ab10445) at 1/1000 dilution

      Lane 1 : Membrane lysates from wild-type primary human osteoblasts (HOB)
      Lane 2 : Membrane lysates from TG2-transfected HOB cells (HOB-TG14)

      Lysates/proteins at 30 µg per lane.

      Secondary
      Rabbit anti-goat-HRP
      Developed using the ECL technique

      Performed under reducing conditions.

      Image from Scarpellini A et al, J Biol Chem. 2009 Jul 3;284(27):18411-23. Epub 2009 Apr 27, Fig 3. DOI 10.1074/jbc.M109.012948

    References for Anti-Transglutaminase 2 antibody (ab10445)

    This product has been referenced in:
    • Scarpellini A  et al. Heparan Sulfate Proteoglycans Are Receptors for the Cell-surface Trafficking and Biological Activity of Transglutaminase-2. J Biol Chem 284:18411-23 (2009). WB ; Mouse . Read more (PubMed: 19398782) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"