• Product name
    Anti-Transglutaminase 2 antibody
    See all Transglutaminase 2 primary antibodies
  • Description
    Rabbit polyclonal to Transglutaminase 2
  • Specificity
    This antibody recognises transglutaminase enzyme. MW is approximately 82kDa. This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-4).
  • Tested applications
    Suitable for: WB, ELISA, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Guinea pig, Human
  • Immunogen

    Full length native protein (purified) (Guinea Pig).

  • Positive control
    • IHC-P: liver sections WB: human fibroblasts (see review), guinea pig liver lysate



Our Abpromise guarantee covers the use of ab421 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/200 - 1/5000.
ELISA 1/300. 1/300 (See Abreview).
IHC-P 1/50 - 1/500.
IHC-Fr Use at an assay dependent concentration. PubMed: 21359202
ICC/IF 1/1000.


  • Function
    Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • Sequence similarities
    Belongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all


  • This was submitted as part of a review by Dr O Fromigue

  • ICC/IF image of ab421 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab421, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Immnohistochemical analysis of murine intestine tissue treated with poly(I:C), staining Transglutaminase 2 with ab421.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.3% Triton X-100 for 10 min at RT, then blocked in 0.3% Triton X-100 and 5% goat serum in PBS. Samples were incubated with primary antibody (1/250) overnight at 4°C. An AlexaFluor®488-conjugated anti-rabbit IgG was used as the secondary antibody.


This product has been referenced in:
  • Pandya CD  et al. Transglutaminase 2 overexpression induces depressive-like behavior and impaired TrkB signaling in mice. Mol Psychiatry 22:745-753 (2017). IHC . Read more (PubMed: 27620841) »
  • Cao J & Huang W Compensatory Increase of Transglutaminase 2 Is Responsible for Resistance to mTOR Inhibitor Treatment. PLoS One 11:e0149388 (2016). WB . Read more (PubMed: 26872016) »

See all 16 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Human Cell lysate - other (lung)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%


Verified customer

Submitted Aug 08 2005

Thank you for your enquiry and your patience in awaiting a response. This product is raised against tissue TGM (TGM2). I have updated the data sheet to reflect this and added data base links relating to the correct target. If you have nay further quest...

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This antibody was tested in WB with lysates from guinea pig liver. It has never specifically been tested with human squamous epithelial samples. Ab421 does cross-react with Human and it is recommended for use at a dilution of 1/200 - 1/5000.

Ab421 was raised using guinea pig tissue Tgase (full length native protein (purified)). This antibody cross-reacts with Factor XIIIA, Keratinocyte Tgase (TG-3), and Prostate Tgase (TG-3). If you have any more questions please contact us again.

This antibody has been tested and characterized on formalin-fixed paraffin-embedded liver tissue. We do not have data on frozen sections, however it is likely that this product will work on acetone or ethanol-fixed tissue section as well.

This antibody recognises cellular/tissue transglutaminase. The immunogen used was purified transglutaminase from guinea pig liver. 'TG1' referred to this being the first transglutaminase antibody to be added to our catalog, not the isoform of the a...

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