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Rabbit polyclonal to Transglutaminase 2
Synthetic peptide from center region of human TGM2 conjugated to KLH
K562 cell lysate
Human lung carcinoma tissue
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Concentration information loading...
Ammonium Sulphate Precipitation
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 77 kDa (predicted molecular weight: 77 kDa).
1/10 - 1/50.
1/10 - 1/50.
Catalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
Belongs to the transglutaminase superfamily. Transglutaminase family.
Information by UniProt
ALPHA SUBUNIT antibody
C polypeptide antibody
EC 22.214.171.124 antibody
Western blot - Anti-Transglutaminase 2 antibody (ab71099)
All lanes : Anti-Transglutaminase 2 antibody (ab71099) Lane 1 : HUVEC lysate Lane 2 : K562 cell lysate Lysates/proteins at 35 µg per lane. Predicted band size : 77 kDa
Immunocytochemistry/ Immunofluorescence - Anti-Transglutaminase 2 antibody (ab71099)
Immunofluorescence image of U251 cells labelling Transglutaminase 2 with ab71099. U251 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ab71099 (1:25, 1 h at 37℃). Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used as the secondary antibody (1:400, 50 min at 37℃). Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37℃) and nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). Transglutaminase 2 immunoreactivity is localised to the cytoplasm.
Western blot - Transglutaminase 2 antibody (ab71099)
Anti-Transglutaminase 2 antibody (ab71099) at 1/60 dilution + K562 cell lysate at 35 µg
Predicted band size : 77 kDa Observed band size : 77 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Transglutaminase 2 antibody (ab71099)
Formalin-fixed and paraffin-embedded human lung carcinoma tissue labelled with ab71099 at 1/10 diution followed by peroxidase conjugation to the secondary antibody and DAB staining.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"