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Recombinant full length human Transglutaminase 2.
ab80563 is excellent for staining of endothelial cells in murine tissues.
Our Abpromise guarantee covers the use of ab80563 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 5 - 10 µg/ml. Predicted molecular weight: 77 kDa.
Use for 2 hours at room temperature.
|IP||Use at 10 µg/mg of lysate.
|IHC-P||Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Use for 30 minutes at room temperature. Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at room temperature for 20 minutes.
ab80563 has not yet been referenced specifically in any publications.