Anti-Transglutaminase 2 antibody [EP2957] (ab109200)

Overview

  • Product nameAnti-Transglutaminase 2 antibody [EP2957]
    See all Transglutaminase 2 primary antibodies
  • Description
    Rabbit monoclonal [EP2957] to Transglutaminase 2
  • Tested applicationsSuitable for: WB, IP, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • U87-MG, A549 and HUVEC cell lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109200 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 77 kDa.
IP 1/10 - 1/100.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.

Target

  • FunctionCatalyzes the cross-linking of proteins and the conjugation of polyamines to proteins.
  • Sequence similaritiesBelongs to the transglutaminase superfamily. Transglutaminase family.
  • Information by UniProt
  • Database links
  • Alternative names
    • ALPHA SUBUNIT antibody
    • C polypeptide antibody
    • EC 2.3.2.13 antibody
    • epididymis secretory protein Li 45 antibody
    • G alpha h antibody
    • G[a]h antibody
    • Gh CLASS G ALPHA h antibody
    • GNAH antibody
    • GNAH G PROTEIN antibody
    • H POLYPEPTIDE antibody
    • HEL-S-45 antibody
    • Protein glutamine gamma glutamyltransferase 2 antibody
    • Protein-glutamine gamma-glutamyltransferase 2 antibody
    • TG 2 antibody
    • TG(C) antibody
    • TG2 antibody
    • TGase C antibody
    • TGase H antibody
    • TGase-2 antibody
    • TgaseII antibody
    • TGC antibody
    • TGC GUANINE NUCLEOTIDE BINDING PROTEIN antibody
    • TGM2 antibody
    • TGM2_HUMAN antibody
    • Tissue transglutaminase antibody
    • Transglutaminase 2 antibody
    • Transglutaminase 2 C polypeptide antibody
    • Transglutaminase C antibody
    • Transglutaminase H antibody
    • Transglutaminase-2 antibody
    • tTG antibody
    • tTGas antibody
    see all

Anti-Transglutaminase 2 antibody [EP2957] images

  • All lanes : Anti-Transglutaminase 2 antibody [EP2957] (ab109200) at 1/10000 dilution

    Lane 1 : U87-MG cell lysate
    Lane 2 : A549 cell lysate
    Lane 3 : HUVEC cell lysate

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 77 kDa
  • ab109200, at 1/100 dilution, staining Transglutaminase 2 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
  • ab109200 staining Transglutaminase 2 in mouse kidney and heart tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Tween-20 and blocked with 5% BSA for 12 hours at 4°C. Samples were incubated with primary antibody (1/500 in PBS-T + 5% BSA) for 12 hours at 4°C. An undiluted Alexa Fluor® 488-conjugated goat anti-rabbit IgG (H+L) polyclonal was used as the secondary antibody.

    See Abreview

References for Anti-Transglutaminase 2 antibody [EP2957] (ab109200)

ab109200 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Frozen sections)
Blocking step BSA as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Sample Mouse Tissue sections (Kidney, Heart)
Specification Kidney, Heart
Permeabilization Yes - 0.1% Tween-20
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jun 02 2014

Thank you for your enquiry.

I can confirm that ab109200 Transglutaminase 2 antibody [EP2957] has the following alignments to the isoforms of the protein:

Isoform 1 100%
isoform 2 and 3 26%

Therefore, the antibody i...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"