Anti-TGE antibody [B5D] (ab53236)
Key features and details
- Mouse monoclonal [B5D] to TGE
- Suitable for: WB
- Isotype: IgG1
Overview
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Product name
Anti-TGE antibody [B5D]
See all TGE primary antibodies -
Description
Mouse monoclonal [B5D] to TGE -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Immunogen
Recombinant full length protein corresponding to Human TGE.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituents: 1.21% Tris, 0.75% Glycine, 2% Sucrose -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
B5D -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab53236 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/200 - 1/1000. Predicted molecular weight: 77 kDa.
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Notes |
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WB
1/200 - 1/1000. Predicted molecular weight: 77 kDa. |
Target
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Function
Catalyzes the calcium-dependent formation of isopeptide cross-links between glutamine and lysine residues in various proteins, as well as the conjugation of polyamines to proteins. Involved in the formation of the cornified envelope (CE), a specialized component consisting of covalent cross-links of proteins beneath the plasma membrane of terminally differentiated keratinocytes. Catalyzes small proline-rich proteins (SPRR1 and SPRR2) and LOR cross-linking to form small interchain oligomers, which are further cross-linked by TGM1 onto the growing CE scaffold (By similarity). In hair follicles, involved in cross-linking structural proteins to hardening the inner root sheath. -
Sequence similarities
Belongs to the transglutaminase superfamily. Transglutaminase family. -
Post-translational
modificationsActivated by proteolytic processing. In vitro activation is commonly achieved by cleavage with dispase, a neutral bacterial protease. Dispase cleavage site was proposed to lie between Ser-470 and Ser-471 (PubMed:8099584) or between Pro-465 and Phe-466 (PubMed:16565075). Physiological activation may be catalyzed by CTSL and, to a lesser extent, by CTSS, but not by CTSB, CTSD nor CTSV (PubMed:16565075). - Information by UniProt
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Alternative names
- E polypeptide protein glutamine gamma glutamyltransferase antibody
- MGC126249 antibody
- MGC126250 antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab53236 has not yet been referenced specifically in any publications.