The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
RelevanceTransglutaminases are a family of structurally and functionally related enzymes that stabilize protein assemblies through the formation of gamma glutamyl epsilon lysine crosslinks. They all have the same amino acid sequence at their active sites and their activity is calcium dependent.