Anti-Transition Protein 1 antibody (ab73135)

Overview

  • Product name
    Anti-Transition Protein 1 antibody
    See all Transition Protein 1 primary antibodies
  • Description
    Rabbit polyclonal to Transition Protein 1
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human Transition Protein 1.

  • Positive control
    • This antibody gave a positive signal in Human normal testis FFPE section.

Properties

Applications

Our Abpromise guarantee covers the use of ab73135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • IHC image of Transition Protein 1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References

This product has been referenced in:
  • Han H  et al. Testicular Characteristics and the Block to Spermatogenesis in Mature Hinny. Asian-Australas J Anim Sci 29:793-800 (2016). IF . Read more (PubMed: 26954128) »
  • Yassine S  et al. Dpy19l2-deficient globozoospermic sperm display altered genome packaging and DNA damage that compromises the initiation of embryo development. Mol Hum Reprod 21:169-85 (2015). Read more (PubMed: 25354700) »

See all 3 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample
Mouse Tissue sections (Testis)
Specification
Testis
Permeabilization
No
Fixative
Bouin's solution
Username

Abcam user community

Verified customer

Submitted May 06 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (Testes)
Loading amount
1 µg
Specification
Testes
Gel Running Conditions
Reduced Non-Denaturing (Native) (NuPage 4-12% Bis-Tris)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

I am very pleased to hear you would like to accept our offer and test ab73135 in Western Blot. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Western Blot. ...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up