Anti-Transition Protein 1 antibody (ab73135)

Overview

  • Product nameAnti-Transition Protein 1 antibody
    See all Transition Protein 1 primary antibodies
  • Description
    Rabbit polyclonal to Transition Protein 1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Dog, Pig
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 to the C-terminus of Human Transition Protein 1.

  • Positive control
    • This antibody gave a positive signal in Human normal testis FFPE section.

Properties

Applications

Our Abpromise guarantee covers the use of ab73135 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionIn the elongating spermatids of mammals, the conversion of nucleosomal chromatin to the compact, non-nucleosomal form found in the sperm nucleus is associated with the appearance of a small set of basic chromosomal transition proteins.
  • Tissue specificityTestis.
  • Sequence similaritiesBelongs to the nuclear transition protein 1 family.
  • Cellular localizationNucleus. Chromosome.
  • Information by UniProt
  • Database links
  • Alternative names
    • Spermatid nuclear transition protein 1 antibody
    • Spermatid nuclear transition protein1 antibody
    • STP 1 antibody
    • STP-1 antibody
    • STP1 antibody
    • STP1_HUMAN antibody
    • TNP 1 antibody
    • TNP1 antibody
    • TP 1 antibody
    • TP-1 antibody
    • TP1 antibody
    • Transition protein 1 (during histone to protamine replacement) antibody
    • Transition protein1 antibody
    see all

Anti-Transition Protein 1 antibody images

  • IHC image of Transition Protein 1 staining in Human normal testis FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73135, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX

References for Anti-Transition Protein 1 antibody (ab73135)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1.5% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate buffer
Sample Mouse Tissue sections (Testis)
Specification Testis
Permeabilization No
Fixative Bouin's solution
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Abcam user community

Verified customer

Submitted May 06 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Testes)
Loading amount 1 µg
Specification Testes
Gel Running Conditions Reduced Non-Denaturing (Native) (NuPage 4-12% Bis-Tris)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Abcam user community

Verified customer

Submitted Feb 08 2013

I am very pleased to hear you would like to accept our offer and test ab73135 in Western Blot. This code will give you the VALUE of this product off an order before the expiration date. To redeem this offer, please submit an Abreview for Western Blot. ...

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