Anti-Transketolase antibody [7H1AA1] (ab112997)

Overview

  • Product nameAnti-Transketolase antibody [7H1AA1]
    See all Transketolase primary antibodies
  • Description
    Mouse monoclonal [7H1AA1] to Transketolase
  • SpecificityShotgun immunization of human HeLa cell lysates into mice. Targets were determined by mass spectrometry and validated by WB, ICC, ELISA pair and other techniques.
  • Tested applicationsSuitable for: IHC-P, ICC/IF, IP, WB, In-Cell ELISA, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Hela cell cytosol fraction

  • Positive control
    • HeLa and HepG2 cell lysates; HL60 and HeLa cells. Human normal colon FFPE tissue.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

     

    Alternative versions:

    Anti-Transketolase antibody (Alexa Fluor® 647) [7H1AA1] (ab198134)

    Anti-Transketolase antibody (HRP) [7H1AA1] (ab198310)

Properties

Applications

Our Abpromise guarantee covers the use of ab112997 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 1 µg/ml.
IP Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 68 kDa.
In-Cell ELISA Use a concentration of 1 µg/ml.
Flow Cyt Use a concentration of 1 µg/ml. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

Anti-Transketolase antibody [7H1AA1] images

  • All lanes : Anti-Transketolase antibody [7H1AA1] (ab112997) at 1 µg/ml

    Lane 1 : HepG2 Cell Lysate
    Lane 2 : HeLa Cell Lysate

    Lysates/proteins at 20 µg per lane.


    Predicted band size : 68 kDa
  • ab112997 at 1ug/ml staining Transketolase in HL60 cells by Flow Cytometry (blue). Isotype control antibody (red).
  • ab112997 at 1ug/ml staining Transketolase in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI (blue). Non specific fluorescence was reduced using a signal enhancer.
  • IHC image of Transketolase staining in Human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab112997, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-Transketolase antibody [7H1AA1] (ab112997)

ab112997 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your interest in Abcam products.

To our knowledge, ab112997 has not been tested in Dog. However the immunogen sequence of ab112997 is quite homologous with Dog species so this ab most likely would cross react. I would e...

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Thank you for contacting Abcam regarding ab112997.


Our TKT antibody was developed from a shotgun project, thus we do not exactly know the epitope of the antibody. The antibody was validated with Hela cells which express both TKT and TKT...

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Thank you for your inquiry. We advise our customers to store this antibody at 4C. We do have data that storage at 4C will not affect the quality of this antibody. It might well be possible to freeze this antibody without experiencing quality loss and I...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"