Anti-Transketolase antibody (ab56199)

Overview

  • Product nameAnti-Transketolase antibody
    See all Transketolase primary antibodies
  • Description
    Rabbit polyclonal to Transketolase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to C terminal residues of mouse Transketolase

Properties

Applications

Our Abpromise guarantee covers the use of ab56199 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: Use at an assay dependent concentration. Predicted molecular weight: 68 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    Anti-Transketolase antibody images

    • Anti-Transketolase antibody (ab56199) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 68 kDa
      Observed band size : 74 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 32 kDa,37 kDa,50 kDa. We are unsure as to the identity of these extra bands.

      Exposure time : 16 minutes

    References for Anti-Transketolase antibody (ab56199)

    ab56199 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Tissue lysate - whole (brain)
    Loading amount 15 µg
    Specification brain
    Gel Running Conditions Reduced Denaturing
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Sep 03 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"