Anti-Translation factor GUF1, mitochondrial antibody - N-terminal (ab171161)

Overview

  • Product name
    Anti-Translation factor GUF1, mitochondrial antibody - N-terminal
  • Description
    Rabbit polyclonal to Translation factor GUF1, mitochondrial - N-terminal
  • Tested applications
    Suitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Translation factor GUF1, mitochondrial aa 190-220 (N terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q8N442

  • Positive control
    • Human prostate carcinoma tissue; HepG2 cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab171161 is purified through a protein A column, followed by peptide affinity purification.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab171161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/100 - 1/500. Predicted molecular weight: 74 kDa.

Target

  • Function
    Promotes mitochondrial protein synthesis. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Binds to mitochondrial ribosomes in a GTP-dependent manner.
  • Sequence similarities
    Belongs to the GTP-binding elongation factor family. LepA subfamily.
  • Cellular localization
    Mitochondrion inner membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • EF-4 antibody
    • Elongation factor 4 homolog antibody
    • GTPase GUF1 antibody
    • GUF1 antibody
    • GUF1_HUMAN antibody
    • mitochondrial antibody
    • Ribosomal back-translocase antibody
    • Translation factor guf1 antibody
    see all

Images

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human prostate carcinoma tissue labeling Translation factor GUF1, mitochondrial with ab171161 at 1/10 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Anti-Translation factor GUF1, mitochondrial antibody - N-terminal (ab171161) at 1/100 dilution + HepG2 cell line lysate at 35 µg
    Developed using the ECL technique

    Predicted band size : 74 kDa

References

ab171161 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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