Overview

  • Product name
  • Description
    Rabbit polyclonal to Translin
  • Specificity
    Detects endogenous levels of total translin protein.
  • Tested applications
    Suitable for: ICC/IF, WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from internal sequence of human translin.

Properties

Applications

Our Abpromise guarantee covers the use of ab71775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB 1/500 - 1/1000. Predicted molecular weight: 26 kDa.
ELISA 1/40000.
IHC-P Use at an assay dependent concentration.

Target

Images

  • All lanes : Anti-Translin antibody (ab71775) at 1/1000 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate with blocking peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 26 kDa
  • Immunohistochemical staining of paraffin-embedded human brain tissue using translin antibody (ab71775) at 1/100 dilution. Staining performed in absence (left) and presence (right) of blocking peptide.
  • ICC/IF image of ab71775 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71775, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

ab71775 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (NT2)
Loading amount
75000 cells
Specification
NT2
Gel Running Conditions
Reduced Denaturing (NuPAGE® Novex 4-12% Bis-Tris Gel 1.0 mm)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Nasser Almobadel

Verified customer

Submitted Mar 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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