SpecificityThis antibody recognizes recombinant proteins containing either an amino or carboxyl terminal HHHHHHGS epitope tag, which is encoded by most His6 tag expression vectors.
The clone number has been updated from (0.T.59) to (4D11) both clone numbers name the same antibody clone.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesICC/IF: Use at an assay dependent dilution to detect His tagged proteins in transfected cells.
WB: Use at a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionTRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
Sequence similaritiesBelongs to the TRAP-alpha family.
DomainShows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.