Overview

  • Product name
  • Description
    Rabbit polyclonal to TRAP alpha
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within internal sequence amino acids 107-156 (FTNKGTEDFI VESLDASFRY PQDYQFYIQN FTALPLNTVV PPQRQATFEY) of Human TRAP alpha (NP_003135).

  • Positive control
    • HepG2 cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab104718 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 32 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    TRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
  • Sequence similarities
    Belongs to the TRAP-alpha family.
  • Domain
    Shows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.
  • Cellular localization
    Endoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781N23103 antibody
    • FLJ14232 antibody
    • FLJ22100 antibody
    • FLJ23034 antibody
    • FLJ78242 antibody
    • FLJ93042 antibody
    • PGP35 antibody
    • Signal sequence receptor alpha antibody
    • Signal sequence receptor alpha subunit antibody
    • Signal sequence receptor subunit alpha antibody
    • SSR 1 antibody
    • SSR alpha antibody
    • SSR-alpha antibody
    • SSR1 antibody
    • SSRA_HUMAN antibody
    • SSRalpha antibody
    • Translocon associated protein alpha antibody
    • Translocon associated protein alpha subunit antibody
    • Translocon associated protein subunit alpha antibody
    • Translocon-associated protein subunit alpha antibody
    • TRAP a antibody
    • TRAP-alpha antibody
    • TRAPa antibody
    • TRAPalpha antibody
    see all

Anti-TRAP alpha antibody images

  • Anti-TRAP alpha antibody (ab104718) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Predicted band size : 32 kDa

References for Anti-TRAP alpha antibody (ab104718)

ab104718 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
Western blot
Sample
Xenopus laevis Cell lysate - other (CSF egg extract)
Loading amount
1 cells
Specification
CSF egg extract
Gel Running Conditions
Reduced Denaturing (12% Acrylamide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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Verified customer

Submitted Mar 25 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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