Overview

  • Product nameAnti-TRAP alpha antibody
    See all TRAP alpha primary antibodies
  • Description
    Mouse polyclonal to TRAP alpha
  • Tested applicationsSuitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human TRAP alpha protein (AAH07710.1).

  • Positive control
    • HeLa cell lysate. A-431 cell lysate. Cell lysate from transfected 293T cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab88644 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
Flow Cyt Use at an assay dependent concentration. ab37355-Mouse polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • FunctionTRAP proteins are part of a complex whose function is to bind calcium to the ER membrane and thereby regulate the retention of ER resident proteins. May be involved in the recycling of the translocation apparatus after completion of the translocation process or may function as a membrane-bound chaperone facilitating folding of translocated proteins.
  • Sequence similaritiesBelongs to the TRAP-alpha family.
  • DomainShows a remarkable charge distribution with the N-terminus being highly negatively charged, and the cytoplasmic C-terminus positively charged.
  • Cellular localizationEndoplasmic reticulum membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • DKFZp781N23103 antibody
    • FLJ14232 antibody
    • FLJ22100 antibody
    • FLJ23034 antibody
    • FLJ78242 antibody
    • FLJ93042 antibody
    • PGP35 antibody
    • Signal sequence receptor alpha antibody
    • Signal sequence receptor alpha subunit antibody
    • Signal sequence receptor subunit alpha antibody
    • SSR 1 antibody
    • SSR alpha antibody
    • SSR-alpha antibody
    • SSR1 antibody
    • SSRA_HUMAN antibody
    • SSRalpha antibody
    • Translocon associated protein alpha antibody
    • Translocon associated protein alpha subunit antibody
    • Translocon associated protein subunit alpha antibody
    • Translocon-associated protein subunit alpha antibody
    • TRAP a antibody
    • TRAP-alpha antibody
    • TRAPa antibody
    • TRAPalpha antibody
    see all

Anti-TRAP alpha antibody images

  • ab88644 detecting TRAP alpha in TRAP alpha-expressing 293 cells (Green) compared with negative control 293 cells (Black) by flow cytometry.
  • Anti-TRAP alpha antibody (ab88644) at 1 µg/ml + HeLa cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
    Additional bands at : ~45 kDa. We are unsure as to the identity of these extra bands.
  • Anti-TRAP alpha antibody (ab88644) at 1 µg/ml + A-431 cell lysate at 50 µg

    Predicted band size : 32 kDa
    Observed band size : 32 kDa
  • All lanes : Anti-TRAP alpha antibody (ab88644) at 1 µg/ml

    Lane 1 : cell lysate from TRAP alpha transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 32 kDa
    Observed band size : 32 kDa

References for Anti-TRAP alpha antibody (ab88644)

ab88644 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"