Anti-TRAP1 antibody (ab151239)
Key features and details
- Rabbit polyclonal to TRAP1
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-TRAP1 antibody
See all TRAP1 primary antibodies -
Description
Rabbit polyclonal to TRAP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Cow -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 392-704 of Human TRAP1 (Uniprot: Q12931).
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Positive control
- 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 89.99% PBS, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151239 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 80 kDa.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 80 kDa. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Chaperone that expresses an ATPase activity. -
Tissue specificity
Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Cellular localization
Mitochondrion. - Information by UniProt
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Database links
- Entrez Gene: 416666 Chicken
- Entrez Gene: 514472 Cow
- Entrez Gene: 10131 Human
- Entrez Gene: 68015 Mouse
- Entrez Gene: 287069 Rat
- Omim: 606219 Human
- SwissProt: Q2TBI4 Cow
- SwissProt: Q12931 Human
see all -
Alternative names
- Heat shock protein 75 kDa antibody
- Heat shock protein 75 kDa, mitochondrial antibody
- HSP 75 antibody
see all
Images
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All lanes : Anti-TRAP1 antibody (ab151239) at 1/500 dilution
Lane 1 : WT 293T cell extracts
Lane 2 : TRAP1 Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 80 kDa -
All lanes : Anti-TRAP1 antibody (ab151239) at 1/1500 dilution
Lane 1 : HepG2 whole cell lysate
Lane 2 : MOLT4 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 80 kDa
7.5% SDS PAGE -
Anti-TRAP1 antibody (ab151239) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg
Predicted band size: 80 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells labeling TRAP1 with ab151239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab151239 has been referenced in 3 publications.
- Li X et al. Epigenetic regulation of NfatC1 transcription and osteoclastogenesis by nicotinamide phosphoribosyl transferase in the pathogenesis of arthritis. Cell Death Discov 5:62 (2019). PubMed: 30774990
- Lin MH et al. Immunological evaluation of a novel HLA-A2 restricted phosphopeptide of tumor associated Antigen, TRAP1, on cancer therapy. Vaccine X 1:100017 (2019). PubMed: 31384738
- Wang Y et al. Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro. Int J Mol Med 36:1487-96 (2015). WB . PubMed: 26500047