Overview

  • Product name
  • Description
    Rabbit polyclonal to TRAP1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 392-704 of Human TRAP1 (Uniprot: Q12931).

  • Positive control
    • 293T, A431, H1299, HeLaS3, HepG2, MOLT4, Raji and Mouse brain whole cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151239 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 80 kDa.
ICC/IF 1/100 - 1/1000.

Target

Images

  • All lanes : Anti-TRAP1 antibody (ab151239) at 1/1500 dilution

    Lane 1 : HepG2 whole cell lysate
    Lane 2 : MOLT4 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Anti-TRAP1 antibody (ab151239) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size: 80 kDa



    7.5% SDS PAGE
  • Immunofluorescence analysis of methanol-fixed HeLa cells labeling TRAP1 with ab151239 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.

References

This product has been referenced in:
  • Wang Y  et al. Overexpression of mitochondrial Hsp75 protects neural stem cells against microglia-derived soluble factor-induced neurotoxicity by regulating mitochondrial permeability transition pore opening in vitro. Int J Mol Med 36:1487-96 (2015). WB . Read more (PubMed: 26500047) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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