Recombinant
RabMAb

Anti-TRAP1 antibody [EPR5381] (ab109323)

Overview

  • Product name
    Anti-TRAP1 antibody [EPR5381]
    See all TRAP1 primary antibodies
  • Description
    Rabbit monoclonal [EPR5381] to TRAP1
  • Tested applications
    Suitable for: WB, IHC-P, ICCmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human TRAP1.

  • Positive control
    • WB: K562, HL60, 293T and HeLa cell lysates IHC-P: Human kidney and liver tissues
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab109323 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 75 kDa (predicted molecular weight: 80 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/250 - 1/5000.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Chaperone that expresses an ATPase activity.
    • Tissue specificity
      Found in skeletal muscle, liver, heart, brain, kidney, pancreas, lung and placenta.
    • Sequence similarities
      Belongs to the heat shock protein 90 family.
    • Cellular localization
      Mitochondrion.
    • Information by UniProt
    • Database links
    • Alternative names
      • Heat shock protein 75 kDa antibody
      • Heat shock protein 75 kDa, mitochondrial antibody
      • HSP 75 antibody
      • HSP75 antibody
      • HSP90L antibody
      • mitochondrial antibody
      • TNF receptor associated protein 1 antibody
      • TNFR-associated protein 1 antibody
      • TRAP-1 antibody
      • Trap1 antibody
      • TRAP1_HUMAN antibody
      • Tumor necrosis factor type 1 receptor-associated protein antibody
      see all

    Anti-TRAP1 antibody [EPR5381] images

    • All lanes : Anti-TRAP1 antibody [EPR5381] (ab109323) at 1/10000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : 293T cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 80 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab109323, at a 1/100 dilution, staining TRAP1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    References for Anti-TRAP1 antibody [EPR5381] (ab109323)

    ab109323 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab109323.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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