Overview

  • Product nameAnti-TRAP220/MED1 antibody
    See all TRAP220/MED1 primary antibodies
  • Description
    Rabbit polyclonal to TRAP220/MED1
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide derived from internal region of human TRAP220/MED1

  • Positive control
    • Human breast carcinoma tissue. Extracts from Jurkat cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab64965 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/10000.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Tissue specificityUbiquitously expressed.
  • Sequence similaritiesBelongs to the Mediator complex subunit 1 family.
  • Post-translational
    modifications
    Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activator-recruited cofactor 205 kDa component antibody
    • ARC205 antibody
    • CRSP1 antibody
    • CRSP200 antibody
    • DRIP205 antibody
    • DRIP230 antibody
    • MED1 antibody
    • MED1_HUMAN antibody
    • Mediator complex subunit 1 antibody
    • Mediator of RNA polymerase II transcription subunit 1 antibody
    • p53 regulatory protein RB18A antibody
    • PBP antibody
    • Peroxisome proliferator-activated receptor-binding protein antibody
    • PPAR binding protein antibody
    • PPAR-binding protein antibody
    • PPARBP antibody
    • PPARGBP antibody
    • RB18A antibody
    • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
    • Thyroid receptor-interacting protein 2 antibody
    • TR-interacting protein 2 antibody
    • Trap220 antibody
    • TRIP-2 antibody
    • TRIP2 antibody
    • Vitamin D receptor-interacting protein complex component DRIP205 antibody
    see all

Anti-TRAP220/MED1 antibody images

  • All lanes : Anti-TRAP220/MED1 antibody (ab64965) at 1/500 dilution

    Lane 1 : extracts from Jurkat cells, without immunising peptide
    Lane 2 : extracts from Jurkat cells, with immunising peptide


    Predicted band size : 180 kDa
    Observed band size : 170 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using 1/50 ab64965, after antigen retrieval by boiling in a pressure cooker for 2-3 min. Polymer system was used for signal enhancing and DAB staining was performed for visualising the signal.

References for Anti-TRAP220/MED1 antibody (ab64965)

This product has been referenced in:
  • Villar J  et al. Androgen-induced activation of gonadotropin-regulated testicular RNA helicase (GRTH/Ddx25) transcription: essential role of a nonclassical androgen response element half-site. Mol Cell Biol 32:1566-80 (2012). Read more (PubMed: 22331472) »
  • Hulf T  et al. Epigenetic-induced repression of microRNA-205 is associated with MED1 activation and a poorer prognosis in localized prostate cancer. Oncogene : (2012). Read more (PubMed: 22869146) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (U2OS cells)
Gel Running Conditions Reduced Denaturing (10% Gel)
Loading amount 50 µg
Specification U2OS cells
Blocking step Licor Blocking Buffer as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 25°C
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Submitted Oct 05 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"