Overview

  • Product name
    Anti-TRAP220/MED1 antibody
    See all TRAP220/MED1 primary antibodies
  • Description
    Rabbit polyclonal to TRAP220/MED1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 400 - 500 of Human TRAP220/MED1.

    (Peptide available as ab92663.)

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293.

Properties

Applications

Our Abpromise guarantee covers the use of ab82461 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 168 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function
      Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • Tissue specificity
      Ubiquitously expressed.
    • Sequence similarities
      Belongs to the Mediator complex subunit 1 family.
    • Post-translational
      modifications
      Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localization
      Nucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator-recruited cofactor 205 kDa component antibody
      • ARC205 antibody
      • CRSP1 antibody
      • CRSP200 antibody
      • DRIP205 antibody
      • DRIP230 antibody
      • MED1 antibody
      • MED1_HUMAN antibody
      • Mediator complex subunit 1 antibody
      • Mediator of RNA polymerase II transcription subunit 1 antibody
      • p53 regulatory protein RB18A antibody
      • PBP antibody
      • Peroxisome proliferator-activated receptor-binding protein antibody
      • PPAR binding protein antibody
      • PPAR-binding protein antibody
      • PPARBP antibody
      • PPARGBP antibody
      • RB18A antibody
      • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
      • Thyroid receptor-interacting protein 2 antibody
      • TR-interacting protein 2 antibody
      • Trap220 antibody
      • TRIP-2 antibody
      • TRIP2 antibody
      • Vitamin D receptor-interacting protein complex component DRIP205 antibody
      see all

    Images

    • All lanes : Anti-TRAP220/MED1 antibody (ab82461) at 1 µg/ml

      Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 168 kDa
      Observed band size : 61 kDa (why is the actual band size different from the predicted?)


      Exposure time : 30 seconds

    References

    ab82461 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab82461.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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