Overview

  • Product nameAnti-TRAP220/MED1 antibody
    See all TRAP220/MED1 primary antibodies
  • Description
    Rabbit polyclonal to TRAP220/MED1
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to a region between residues 1525 and the C-terminus (residue 1581) of human TRAP220/MED1 (NP_004765.2)

  • Positive control
    • Human breast tumor and mouse squamous cell carcinoma tissues.

Properties

Applications

Our Abpromise guarantee covers the use of ab84386 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notesIHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
    • Tissue specificityUbiquitously expressed.
    • Sequence similaritiesBelongs to the Mediator complex subunit 1 family.
    • Post-translational
      modifications
      Phosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
    • Cellular localizationNucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
    • Information by UniProt
    • Database links
    • Alternative names
      • Activator-recruited cofactor 205 kDa component antibody
      • ARC205 antibody
      • CRSP1 antibody
      • CRSP200 antibody
      • DRIP205 antibody
      • DRIP230 antibody
      • MED1 antibody
      • MED1_HUMAN antibody
      • Mediator complex subunit 1 antibody
      • Mediator of RNA polymerase II transcription subunit 1 antibody
      • p53 regulatory protein RB18A antibody
      • PBP antibody
      • Peroxisome proliferator-activated receptor-binding protein antibody
      • PPAR binding protein antibody
      • PPAR-binding protein antibody
      • PPARBP antibody
      • PPARGBP antibody
      • RB18A antibody
      • Thyroid hormone receptor-associated protein complex 220 kDa component antibody
      • Thyroid receptor-interacting protein 2 antibody
      • TR-interacting protein 2 antibody
      • Trap220 antibody
      • TRIP-2 antibody
      • TRIP2 antibody
      • Vitamin D receptor-interacting protein complex component DRIP205 antibody
      see all

    Anti-TRAP220/MED1 antibody images

    • ab84386 at 1/250 dilution staining TRAP220/MED1 in human breast tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
    • ab84386 at 1/250 dilution staining TRAP220/MED1 in mouse squamous cell carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References for Anti-TRAP220/MED1 antibody (ab84386)

    ab84386 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"