Purification notesab60950 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000.
Use at an assay dependent concentration. PubMed: 19122196
1/50 - 1/100.
1/500 - 1/1000. Detects a band of approximately 170 kDa (predicted molecular weight: 180 kDa).
FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
Tissue specificityUbiquitously expressed.
Sequence similaritiesBelongs to the Mediator complex subunit 1 family.
Post-translational modificationsPhosphorylated by MAPK1 or MAPK3 during G2/M phase which may enhance protein stability and promote entry into the nucleolus. Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationNucleus. A subset of the protein may enter the nucleolus subsequent to phosphorylation by MAPK1 or MAPK3.
References for Anti-TRAP220/MED1 (phospho T1457) antibody - ChIP Grade (ab60950)
This product has been referenced in:
Waters KM et al. ERK oscillation-dependent gene expression patterns and deregulation by stress response. Chem Res Toxicol27:1496-503 (2014).
Read more (PubMed: 25068892) »
Zhao W et al. SIMPL enhancement of tumor necrosis factor-a dependent p65-MED1 complex formation is required for mammalian hematopoietic stem and progenitor cell function. PLoS One8:e61123 (2013).
Read more (PubMed: 23630580) »