Overview

  • Product nameAnti-TRAP80 antibody
    See all TRAP80 primary antibodies
  • Description
    Rabbit polyclonal to TRAP80
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 331-361 of Human TRAP80 (NP_004259.3).

  • Positive control
    • MDA-MB231 cell line lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab124176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/500. Predicted molecular weight: 73 kDa.

Target

  • FunctionComponent of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors.
  • Tissue specificityUbiquitous.
  • Involvement in diseaseDefects in MED17 are the cause of microcephaly postnatal progressive with seizures and brain atrophy (MCPHSBA) [MIM:613668]. It is a disorder characterized by postnatal progressive microcephaly and severe developmental retardation associated with cerebral and cerebellar atrophy. Infants manifest swallowing difficulties leading to failure to thrive, jitteriness, poor visual fixation, truncal arching, seizures. There is no acquisition of developmental milestones and patients suffer from marked spasticity and profound retardation. Progressive microcephaly becomes evident few months after birth.
  • Sequence similaritiesBelongs to the Mediator complex subunit 17 family.
  • Post-translational
    modifications
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 77-KD subunit antibody
    • Activator recruited cofactor 77 kDa component antibody
    • Activator-recruited cofactor 77 kDa component antibody
    • ARC77 antibody
    • Cofactor required for Sp1 transcriptional activation subunit 6 antibody
    • cofactor required for Sp1 transcriptional activation, subunit 6, 77kDa antibody
    • CRSP complex subunit 6 antibody
    • CRSP6 antibody
    • CRSP77 antibody
    • DRIP80 antibody
    • med17 antibody
    • MED17_HUMAN antibody
    • Mediator complex subunit 17 antibody
    • Mediator of RNA polymerase II transcription subunit 17 antibody
    • Mediator of RNA polymerase II transcription subunit 17, S cerevisiae, homolog of antibody
    • Thyroid hormone receptor-associated protein complex 80 kDa component antibody
    • Thyroid hormone receptor-associated protein, 80-KD subunit antibody
    • Transcriptional coactivator CRSP77 antibody
    • Trap80 antibody
    • VITAMIN D RECEPTOR-INTERACTING PROTEIN, 80-KD antibody
    • Vitamin D3 receptor-interacting protein complex 80 kDa component antibody
    see all

Anti-TRAP80 antibody images

  • Anti-TRAP80 antibody (ab124176) at 1/100 dilution + MDA MB231 cell line lysate at 35 µg

    Predicted band size : 73 kDa

References for Anti-TRAP80 antibody (ab124176)

ab124176 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 10 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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Submitted Nov 07 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"