Recombinant
RabMAb

Anti-TRAX antibody [EPR13929(B)] (ab181862)

Overview

  • Product name
    Anti-TRAX antibody [EPR13929(B)]
    See all TRAX primary antibodies
  • Description
    Rabbit monoclonal [EPR13929(B)] to TRAX
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human TRAX aa 1-100. The exact sequence is proprietary.
    Database link: Q99598

  • Positive control
    • Human mouse and rat testis lysates; HeLa cells.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR13929(B)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
ICC/IF 1/100.
IP 1/60.

Target

Images

  • Western blot analysis of immunoprecipitation pellet from Human testis lysate immunoprecipitated using ab181862 at 1/60 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

     

  • Immunofluorescent analysis of HeLa cells (-20°C Acetone-fixed) labeling TRAX with ab181862 at 1/100 dilution followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary at 1/200 dilution and counter-stained with DAPI (blue).

  • Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/5000 dilution + Human testis lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution

    Predicted band size : 33 kDa
  • All lanes : Anti-TRAX antibody [EPR13929(B)] (ab181862) at 1/20000 dilution

    Lane 1 : Mouse testis lysate
    Lane 2 : Rat testis lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size : 33 kDa

References

ab181862 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab181862.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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