• Product nameAnti-TRBP antibody
    See all TRBP primary antibodies
  • Description
    Rabbit polyclonal to TRBP
  • SpecificityImmunogen is 100% homolog to human isoforms A and B.
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human, Chimpanzee, Zebrafish
  • Immunogen

    A portion of amino acids 150-200 of human TRBP

  • Positive control
    • Brain lysate



Our Abpromise guarantee covers the use of ab72110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 39 kDa).


  • FunctionRequired for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.
  • Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOQS antibody
    • Prbp antibody
    • RISC loading complex subunit TARBP2 antibody
    • RISC-loading complex subunit tarbp2 antibody
    • TAR (HIV 1) RNA binding protein 2 antibody
    • TAR (HIV) RNA binding protein 2 antibody
    • TAR (HIV) RNA-binding protein TRBP1 antibody
    • TAR RNA Binding Protein 2 antibody
    • TAR RNA-binding protein 2 antibody
    • tarbp2 antibody
    • TARBP2 RISC loading complex RNA binding subunit antibody
    • Trans-activation-responsive RNA-binding protein antibody
    • TRBP antibody
    • TRBP1 antibody
    • TRBP2 antibody
    • TRBP2_HUMAN antibody
    see all

Anti-TRBP antibody images

  • All lanes : Anti-TRBP antibody (ab72110) at 0.5 µg/ml

    Lane 1 : human brain lysate
    Lane 2 : human brain lysate with immunizing peptide

    Predicted band size : 39 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
  • IHC image of ab72110 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab72110, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-TRBP antibody (ab72110)

This product has been referenced in:
  • Kalantari R  et al. Stable association of RNAi machinery is conserved between the cytoplasm and nucleus of human cells. RNA 22:1085-98 (2016). IP ; Human . Read more (PubMed: 27198507) »
  • Bronevetsky Y  et al. T cell activation induces proteasomal degradation of Argonaute and rapid remodeling of the microRNA repertoire. J Exp Med 210:417-32 (2013). WB ; Mouse . Read more (PubMed: 23382546) »

See all 2 Publications for this product

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Brain cortex)
Specification Brain cortex
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C

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Submitted Jun 02 2011