• Product nameAnti-TRBP antibody
    See all TRBP primary antibodies
  • Description
    Rabbit polyclonal to TRBP
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Horse, Human, Monkey
    Predicted to work with: Mouse, Rat, Chicken
  • Immunogen

    Synthetic peptide corresponding to a region between residues 340-390 of human TRBP (NP_599150.1)

  • Positive control
    • Human brain lysate and human brain tissue.



Our Abpromise guarantee covers the use of ab72547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 5 - 8 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 39 kDa).
IHC-P Use a concentration of 5 - 10 µg/ml.


  • FunctionRequired for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. May also play a role in the production of short interfering RNAs (siRNAs) from double-stranded RNA (dsRNA) by DICER1. Binds to the HIV-1 TAR RNA which is located in the long terminal repeat (LTR) of HIV-1, and stimulates translation of TAR-containing RNAs. This is achieved in part at least by binding to and inhibiting EIF2AK2/PKR, thereby reducing phosphorylation and inhibition of EIF2S1/eIF-2-alpha. May also promote translation of TAR-containing RNAs independently of EIF2AK2/PKR.
  • Sequence similaritiesContains 3 DRBM (double-stranded RNA-binding) domains.
  • Cellular localizationCytoplasm. Cytoplasm > perinuclear region. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • LOQS antibody
    • Prbp antibody
    • RISC loading complex subunit TARBP2 antibody
    • RISC-loading complex subunit tarbp2 antibody
    • TAR (HIV 1) RNA binding protein 2 antibody
    • TAR (HIV) RNA binding protein 2 antibody
    • TAR (HIV) RNA-binding protein TRBP1 antibody
    • TAR RNA Binding Protein 2 antibody
    • TAR RNA-binding protein 2 antibody
    • tarbp2 antibody
    • TARBP2 RISC loading complex RNA binding subunit antibody
    • Trans-activation-responsive RNA-binding protein antibody
    • TRBP antibody
    • TRBP1 antibody
    • TRBP2 antibody
    • TRBP2_HUMAN antibody
    see all

Anti-TRBP antibody images

  • All lanes : Anti-TRBP antibody (ab72547) at 6 µg/ml

    Lane 1 : human brain lysate
    Lane 2 : human brain lysate with immunizing peptide

    Predicted band size : 39 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab72547 at 5µg/ml staining TRBP in normal human brain by Immunohistochemistry, Paraffin-embedded tissue.
  • Staining of normal human brain by Immunohistochemistry, Paraffin-embedded tissue using a control rabbit Ig antibody at 5µg/ml.
  • Immunofluorescence analysis of Non-Hodgkin's Lymphoma B cells, staining TRBP with ab72547.

    Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100. Samples were incubated with primary antibody (1/50 in diluent) for 24 hours at 4°C. An AlexaFluor®488-conjugated goat anti-rabbit polyclonal IgG (1/400) was used as the secondary antibody.

    See Abreview

References for Anti-TRBP antibody (ab72547)

This product has been referenced in:
  • Caramuta S  et al. Clinical and functional impact of TARBP2 over-expression in adrenocortical carcinoma. Endocr Relat Cancer 20:551-64 (2013). IHC . Read more (PubMed: 23671264) »
  • Caramuta S  et al. Role of microRNAs and microRNA machinery in the pathogenesis of diffuse large B-cell lymphoma. Blood Cancer J 3:e152 (2013). WB ; Human . Read more (PubMed: 24121164) »

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Adrenocortical carcinoma)
Specification Adrenocortical carcinoma
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH 6.0
Permeabilization No
Blocking step H2O2 as blocking agent for 10 minute(s) · Concentration: 3% · Temperature: RT°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Thank you for contacting us.
The Abreview has been published however the image would have also been nice. I have validated the discount code, please order your next antibody by writing the discount code in additional notes box on the order page. <...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (non-Hodgkin B-cell)
Specification non-Hodgkin B-cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.5% Triton-X in PBS

Abcam user community

Verified customer

Submitted Oct 08 2012

Thank you for contacting us.
Your 100% Abtrial discount code is XXXXXXXX. The expiration date is 07-01-2013. The value of discount code is Euro 370.
Answer to your questions;
- Please place the order either online or by sending email to f...

Read More

I am sorry for the confusion.
The 100% Abreview discount system works in a way that customers need to get the discount code before the purchase of the product. To validate the code they must buy the product and submitt an Abreview.
It is correc...

Read More

Could you please provide me the purchase order number of ab72547? If, the purchase hasn't been done after my offer then I am sorry the offer will not be valid. The discount code offer was for new vial of ab72547 which cannot be used with antibody that ...

Read More

Thank you very much for your interest in Abcam products?

Could you please explain why you only need half a vial of each rather than full vials?

We unfortunately cannot provide antibodies in half volume and we can't even provide free s...

Read More