Our Abpromise guarantee covers the use of ab57752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.


  • FunctionInvolved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  • Tissue specificityExpressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
  • Sequence similaritiesContains 1 P-type (trefoil) domain.
  • Cellular localizationSecreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt
  • Database links
  • Alternative names
    • hITF antibody
    • hP1.B antibody
    • Intestinal trefoil factor antibody
    • ITF antibody
    • mITF antibody
    • OTTMUSP00000021729 antibody
    • P1B antibody
    • Polypeptide P1.B antibody
    • TFF3 antibody
    • TFF3_HUMAN antibody
    • TFI antibody
    • TREFOIL antibody
    • Trefoil factor (intestinal) antibody
    • Trefoil factor 3 antibody
    see all

Anti-Trefoil Factor 3 antibody images

  • Trefoil Factor 3 antibody (ab57752) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab57752 Trefoil Factor 3 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

References for Anti-Trefoil Factor 3 antibody (ab57752)

This product has been referenced in:
  • Hoellen F  et al. Trefoil factor 3 expression in epithelial ovarian cancer exerts a minor effect on clinicopathological parameters. Mol Clin Oncol 5:422-428 (2016). IHC ; Human . Read more (PubMed: 27699037) »
  • Ding A  et al. Impact of NPM, TFF3 and TACC1 on the prognosis of patients with primary gastric cancer. PLoS One 8:e82136 (2013). IHC-P ; Human . Read more (PubMed: 24358147) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

This kit will work with mouse IgM antiobodies as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoc...

Read More

Thank you for contacting us.

ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HT-29)
Loading amount 10 µg
Specification HT-29
Treatment IP
Gel Running Conditions Reduced Denaturing (12)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C

Ms. qianxue wan

Verified customer

Submitted Oct 10 2012

Thank you for your enquiry.
You are correct that both ab108599 and ab57752 are both monoclonal antibodies designed to detect tff3. In additiona they have both been found to detect human tff3 via WB and IHC-P. Other than these characteristics, thar...

Read More