Overview

Properties

Applications

Our Abpromise guarantee covers the use of ab57752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
IHC-P Use a concentration of 3 µg/ml.

Target

  • Function
    Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
  • Tissue specificity
    Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
  • Sequence similarities
    Contains 1 P-type (trefoil) domain.
  • Cellular localization
    Secreted > extracellular space > extracellular matrix > apical lamina.
  • Information by UniProt
  • Database links
  • Alternative names
    • hITF antibody
    • hP1.B antibody
    • Intestinal trefoil factor antibody
    • ITF antibody
    • mITF antibody
    • OTTMUSP00000021729 antibody
    • P1B antibody
    • Polypeptide P1.B antibody
    • TFF3 antibody
    • TFF3_HUMAN antibody
    • TFI antibody
    • TREFOIL antibody
    • Trefoil factor (intestinal) antibody
    • Trefoil factor 3 antibody
    see all

Anti-Trefoil Factor 3 antibody images

  • Trefoil Factor 3 antibody (ab57752) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human small Intestine.
  • Western blot against tagged recombinant protein immunogen using ab57752 Trefoil Factor 3 antibody at 1ug/ml. Predicted band size of immunogen is 33 kDa

References for Anti-Trefoil Factor 3 antibody (ab57752)

This product has been referenced in:
  • Saunders JH  et al. Individual patient oesophageal cancer 3D models for tailored treatment. Oncotarget 8:24224-24236 (2017). IHC-P, ICC/IF ; Human . Read more (PubMed: 27736801) »
  • El-Balat A  et al. TFF3 Expression as Stratification Marker in Borderline Epithelial Tumors of the Ovary. Pathol Oncol Res N/A:N/A (2017). IHC-P ; Human . Read more (PubMed: 28470574) »

See all 6 Publications for this product

Product Wall

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This kit will work with mouse IgM antiobodies as well.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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ab127055 kit can be used with any mouse antibody - this mean the kit would be suitable.

Please note ab49536 is tested and guaranteed to be with mouse species however ab57752 is only tested with human sampl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HT-29)
Loading amount
10 µg
Specification
HT-29
Treatment
IP
Gel Running Conditions
Reduced Denaturing (12)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 27°C
Username

Ms. qianxue wan

Verified customer

Submitted Oct 10 2012

Thank you for your enquiry.
You are correct that both ab108599 and ab57752 are both monoclonal antibodies designed to detect tff3. In additiona they have both been found to detect human tff3 via WB and IHC-P. Other than these characteristics, thar...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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