Recombinant
RabMAb

Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104)

Overview

  • Product name
    Anti-Trefoil Factor 3 antibody [EPR3973]
    See all Trefoil Factor 3 primary antibodies
  • Description
    Rabbit monoclonal [EPR3973] to Trefoil Factor 3
  • Tested applications
    Suitable for: WB, IHC-P, ICC, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human Trefoil Factor 3.

  • Positive control
    • Human uterus cancer and colon cancer lysates; Human colon tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab109104 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 9 kDa.
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC 1/50 - 1/100.
Flow Cyt Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen).
    • Tissue specificity
      Expressed in goblet cells of the intestines and colon (at protein level). Expressed by goblet cells of small and large intestinal epithelia and also by the uterus.
    • Sequence similarities
      Contains 1 P-type (trefoil) domain.
    • Cellular localization
      Secreted > extracellular space > extracellular matrix > apical lamina.
    • Information by UniProt
    • Database links
    • Alternative names
      • hITF antibody
      • hP1.B antibody
      • Intestinal trefoil factor antibody
      • ITF antibody
      • mITF antibody
      • OTTMUSP00000021729 antibody
      • P1B antibody
      • Polypeptide P1.B antibody
      • TFF3 antibody
      • TFF3_HUMAN antibody
      • TFI antibody
      • TREFOIL antibody
      • Trefoil factor (intestinal) antibody
      • Trefoil factor 3 antibody
      see all

    Images

    • All lanes : Anti-Trefoil Factor 3 antibody [EPR3973] (ab109104) at 1/1000 dilution

      Lane 1 : Human uterus cancer lysate
      Lane 2 : Human colon cancer lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 9 kDa
    • Immunohistochemical analysis of Trefoil Factor 3 in paraffin-embedded Human colon tissue using ab109104 at 1/500 dilution.
    • Flow Cytometry analysis of HT-29 (human colorectal adenocarcinoma) cells labeling Trefoil Factor 3 with unpurified ab109104 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    References

    This product has been referenced in:
    • Derakhshan MH  et al. In healthy volunteers, immunohistochemistry supports squamous to columnar metaplasia as mechanism of expansion of cardia, aggravated by central obesity. Gut 64:1705-14 (2015). Read more (PubMed: 25753030) »

    See 1 Publication for this product

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Serum (Mouse Serum)
    Gel Running Conditions
    Reduced Denaturing
    Loading amount
    20 µg
    Specification
    Mouse Serum
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 27°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2016

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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