Anti-Trehalase antibody (ab122600)

Overview

  • Product nameAnti-Trehalase antibody
    See all Trehalase primary antibodies
  • Description
    Rabbit polyclonal to Trehalase
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    antigen sequence: LYQDDKQFVD MPLSIAPEQV LQTFTELSRD HNHSIPREQL QAFVHEHFQA KGQELQPWTP ADWKDSPQFL QKISDAKLRA WAGQLHQLWK KLGKK (Human).

  • Positive control
    • Human small intestine tissue; RT 4, U 251 MG, Human plasma, liver and tonsil lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab122600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionIntestinal trehalase is probably involved in the hydrolysis of ingested trehalose.
  • Involvement in diseaseNote=Deficiency of TREH results in isolated trehalose intolerance that causes gastrointestinal symptoms after ingestion of edible mushrooms.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 37 family.
  • Cellular localizationCell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha alpha trehalase antibody
    • Alpha alpha trehalose glucohydrolase antibody
    • Alpha antibody
    • alpha-trehalase antibody
    • alpha-trehalose glucohydrolase antibody
    • Brush border membrane glycoprotein antibody
    • MGC129621 antibody
    • TRE antibody
    • TREA antibody
    • TREA_HUMAN antibody
    • TREH antibody
    • Trehalase antibody
    see all

Anti-Trehalase antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab122600 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling Trehalase with ab122600. Staining shows moderate luminal membranous positivity in glandular cells.

References for Anti-Trehalase antibody (ab122600)

ab122600 has not yet been referenced specifically in any publications.

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