Overview

  • Product nameTREM1 Human ELISA KitSee all TREM1 kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 70 pg/ml
  • Range
    0.069 ng/ml - 50 ng/ml
  • Recovery

    > 100 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 124.4 106% - 134%
    Serum 125.5 91% - 145%
    Plasma 116 94% - 115%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TREM1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TREM1 in serum, plasma and cell culture supernatants. (Human TREM1 concentration is pretty low in normal serum, it may not be detected in this assay).

    This assay employs an antibody specific for Human TREM1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TREM1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TREM1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TREM1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • RelevanceTREM1 is also known as triggering receptor expressed on myeloid cells 1, and triggering receptor expressed on monocytes 1. TREM1 is a Type I membrane protein that contains an immunoglobulin-like V-type domain. An alternatively spliced protein variant may be secreted. TREM1 is highly expressed on peripheral blood myeloid cells (particularly mature monocytes and granulocytes); TREM1 expression can be further upregulated by bacteria, fungi and lipopolysaccharide. TREM1 has been shown to interact with the adaptor protein DAP12 to stimulate neutrophil and monocyte-mediated inflammatory responses through the triggering and release of pro-inflammatory cytokines and chemokines. TREM1 is thought to amplify inflammatory responses to fungal and bacterial infections and potentiate septic shock.
  • Cellular localizationSecreted and Cell membrane.
  • Alternative names
    • CD354
    • TREM 1
    • Triggering receptor expressed on monocytes 1
    • Triggering receptor expressed on myeloid cells 1
    • Triggering receptor TREM 1
    • Triggering receptor TREM1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100659 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

TREM1 Human ELISA Kit images

  • Representative standard curve using ab100659

  • Representative standard curve using ab100659

Protocols

References for TREM1 Human ELISA Kit (ab100659)

ab100659 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email.

I can confirm that you will find all of the available QC data about serum and plasma near the end of the protocol booklet including spiking&recovery and linearity tests.

Furthermore I like to confirm tha...

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The kit ab100659 TREM1 Human ELISA Kit is suitable to quantify the soluble form of human TREM-1.

Unfortunately, the concentrations used to coat the microplate and the one for the biotynilated antibody ar...

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The report I received from the laboratory tells us that it will be impossible to predict if this ELISA kit can detect the splice variant you are interested in, given the information we have about the antibodies.

From the UniProt page for hum...

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Thank you for your inquiry.

It is recommended to plot the standard curve using a log-log model as stated in the Protocol Booklet. Log-log (common log) is recommended as this model fits most data sets well. However, other graphing models can ...

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Thank you for contacting us and your interest in our products.
The TREM1 Human ELISA Kit (ab100659) uses the diluent A and B in sample preparation of serum/plasma and cell culture medium/urine respectively.
The Assay Diluent A is optimized fo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"