Overview

  • Product name
  • Description
    Goat polyclonal to TREM2
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide:

    C-QVEHSTSRNQET

    , corresponding to internal sequence amino acids 154-165 of Mouse TREM2 (NP_112544.1).

  • Positive control
    • Mouse liver lysate IF/ICC: RAW 246.7 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab95470 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • Tissue specificity
    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • Involvement in disease
    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Images

  • ab95470 stained RAW 246.7 cells. The cells were 100% methanol fixed for 5 minutes at-20°C and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab95470 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was Donkey Anti-Goat IgG H&L (Alexa Fluor® 488) preadsorbed (ab150133) used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.

  • Anti-TREM2 antibody (ab95470) at 0.3 µg/ml + Mouse liver lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa
    Observed band size : 28 kDa (why is the actual band size different from the predicted?)
    Primary incubation was 1 hour.
  • Immunocytochemical immunoflurescence analysis of PFA-fixed human neuroblastoma cell line, labelling TREM2 using ab95470 at a dilution of 1/200 incubated for 2 hours and 24°C in 0.1M PBST with 10% donkey serum. Permeablisation was accomplished using 0.1M PBS with 1% Triton X. Blocking was done with 10% donkey serum in PBST and incubated for 30 minutes at 24°C. The secondary used was a polyclonal donkey anti-goat Alexa Fluor® 568 conjugate.

    See Abreview

References

This product has been referenced in:
  • Jendresen C  et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). Read more (PubMed: 28320424) »
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27662313) »

See all 2 Publications for this product

Customer reviews and Q&As

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain)
Antigen retrieval step
None
Permeabilization
Yes - 0.1xPBS with 1% TritonX100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 17 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Neuroblastoma cell line)
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Neuroblastoma cell line
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Application
Western blot
Sample
Human Serum (Human serum)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Human serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Vielen Dank für Ihre Email.


Wie besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer 123456und sollte Freitag bei Ihnen ankommen.

Ich wünsche Ihnen viel Erfolg ...

Read More

Unser Garantiezeitraum beträgt6 Monate, und eigentlich kann ich bei einer Bestellung vom Januar 2011kein Auge zudrücken.

Da ichIhnen allerdings schon einen Ersatz zugesagt habe und es versäumt habe,den Garantiezeitraum zu erw&au...

Read More

Vielen Dank für Ihre Geduld.

Da der Antikörper nicht so funktioniert wie er sollte, werden wir Ihnen gerne einen kostenlosen Ersatz senden, Ihnen eine Gutschrift ausstellen oder den Antikörper erstatten.

Wir müsste...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up