Anti-TREM2 antibody (ab95470)
Key features and details
- Goat polyclonal to TREM2
- Suitable for: ICC
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-TREM2 antibody
See all TREM2 primary antibodies -
Description
Goat polyclonal to TREM2 -
Host species
Goat -
Tested applications
Suitable for: ICCmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Rat -
Immunogen
Synthetic peptide corresponding to Mouse TREM2 aa 154-165 (internal sequence).
Sequence:C-QVEHSTSRNQET
Database link: Q99NH8 -
Positive control
- ICC: NIH3T3-L1 cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab95470 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95470 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC |
Use a concentration of 10 mg/ml.
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Notes |
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ICC
Use a concentration of 10 mg/ml. |
Target
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Function
May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. -
Tissue specificity
Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord. -
Involvement in disease
Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. -
Sequence similarities
Contains 1 Ig-like V-type (immunoglobulin-like) domain. -
Cellular localization
Secreted and Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 83433 Mouse
- Entrez Gene: 301227 Rat
- SwissProt: Q99NH8 Mouse
- Unigene: 261623 Mouse
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Alternative names
- TREM 2 antibody
- TREM-2 antibody
- TREM2 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence analysis of paraformaldehyde fixed mouse 3T3-L1 cells labeling TREM2 with ab95470 at 10 µg/mL (incubation time, 1 hour). Cells were permeabilized with 0.15% Triton. Alexa Fluor 488 secondary antibody was used at 2µg/mL. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG at 10 µg/mL followed by Alexa Fluor 488 secondary antibody 2 µg/mL.
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Immunofluorescence analysis of paraformaldehyde fixed 3T3-L1 cells, permeabilized with 0.15% Triton. Primary incubation with ab95470 1hr (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 μg/ml) followed by Alexa Fluor 488 secondary antibody (2 μg/ml).
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab95470 has been referenced in 7 publications.
- Sebastian A et al. Single-cell RNA-Seq reveals changes in immune landscape in post-traumatic osteoarthritis. Front Immunol 13:938075 (2022). PubMed: 35967299
- Koronyo-Hamaoui M et al. Peripherally derived angiotensin converting enzyme-enhanced macrophages alleviate Alzheimer-related disease. Brain 143:336-358 (2020). PubMed: 31794021
- Guo Y et al. TREM2 deficiency aggravates a-synuclein-induced neurodegeneration and neuroinflammation in Parkinson's disease models. FASEB J N/A:fj201900992R (2019). PubMed: 31370707
- Ji J et al. The Intra-nuclear SphK2-S1P Axis Facilitates M1-to-M2 Shift of Microglia via Suppressing HDAC1-Mediated KLF4 Deacetylation. Front Immunol 10:1241 (2019). PubMed: 31214192
- Wang ECE et al. A Subset of TREM2+ Dermal Macrophages Secretes Oncostatin M to Maintain Hair Follicle Stem Cell Quiescence and Inhibit Hair Growth. Cell Stem Cell 24:654-669.e6 (2019). PubMed: 30930146
- Jendresen C et al. The Alzheimer's disease risk factors apolipoprotein E and TREM2 are linked in a receptor signaling pathway. J Neuroinflammation 14:59 (2017). PubMed: 28320424
- Raha AA et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . PubMed: 27662313