Overview

  • Product name
    Anti-TREM2 antibody [RM0139-5J46]
    See all TREM2 primary antibodies
  • Description
    Rat monoclonal [RM0139-5J46] to TREM2
  • Specificity
    This antibody detects mouse TREM2, but not TREM1 in Western blotting.
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Mouse recombinant protein of the extracellular EC-domain of TREM2

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    The IgG fraction of culture supernatant was purified by Protein A affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    RM0139-5J46
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/250.
WB 1/500 - 1/1000. Predicted molecular weight: 25 kDa.

Target

  • Function
    May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells.
  • Tissue specificity
    Expressed on macrophages and dendritic cells but not on granulocytes or monocytes. In the CNS strongest expression seen in the basal ganglia, corpus callosum, medulla oblongata and spinal cord.
  • Involvement in disease
    Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL) [MIM:221770]; also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns.
  • Sequence similarities
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • TREM 2 antibody
    • TREM-2 antibody
    • TREM2 antibody
    • TREM2_HUMAN antibody
    • TREM2a antibody
    • TREM2b antibody
    • TREM2c antibody
    • Trggering receptor expressed on myeloid cells 2 antibody
    • Trggering receptor expressed on myeloid cells 2a antibody
    • Triggering receptor expressed on monocytes 2 antibody
    • Triggering receptor expressed on myeloid cells 2 antibody
    see all

Images

  • IHC-Fr image of human blood vessels stained with Ab86491 (1:250). The fixed brain sections were permeabilized using 0.1% PBS-Triton X for 1h. 10% donkey serum was used for 1 hour at 24°C to block protein-protein interactions.  The sections were then incubated with Ab8649 used at 1/250 dilution, overnight at +4°C. The secondary antibody was Alexa Fluor® 568 donkey anti-rat used at 1/1000 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    See Abreview

  • Immunofluorescence analysis of 18 day rat embroyonic hypothalmic neurones labelling TREM2 with ab86491. Embryonic hypothalmic neuronal cells were cultured in a neurobasal media with B27 culture suppliment for three weeks and fixed with 2%PFA. A rat monoclonal Alexa fluoro® 568 (red) secondary was used at a concentration of 1/1000. Synaptophysin was counterstained with an Alexa fluoro® 488 (green) and nuclear DNA was stained with DAPI (blue).

     

    See Abreview

References

This product has been referenced in:
  • Raha AA  et al. Neuroprotective Effect of TREM-2 in Aging and Alzheimer's Disease Model. J Alzheimers Dis N/A:N/A (2016). WB, IHC ; Mouse . Read more (PubMed: 27662313) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Hippocampal neuron)
Permeabilization
Yes - 0.1 M PBS with 1% Triton X
Specification
Hippocampal neuron
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 24°C
Fixative
Paraformaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 21 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Serum (Human Serum)
Gel Running Conditions
Reduced Denaturing (4-12% Bis-Tris)
Loading amount
20 µg
Specification
Human Serum
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 20 2015

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
Sample
Human Tissue sections (Blood vessels)
Specification
Blood vessels
Permeabilization
Yes - 0.1% TritonX in 0.1% PBS
Fixative
Formaldehyde
Username

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Aug 06 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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