Our Abpromise guarantee covers the use of ab20923 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemical analysis of HIV-infected syphilitic skin tissue, staining Treponema pallidum with ab20923.
Antigen retrieval was performed before blocking with normal horse serum and 2% BSA. Samples were incubated with primary antibody overnight at 4°C. A biotinylated horse anti-rabbit IgG was used as the secondary antibody. Staining was detected using AEC.