Anti-Treponema pallidum antibody (Biotin) (ab20465)

Overview

  • Product nameAnti-Treponema pallidum antibody (Biotin)
    See all Treponema pallidum primary antibodies
  • Description
    Rabbit polyclonal to Treponema pallidum (Biotin)
  • ConjugationBiotin
  • SpecificityRecognises all antigens. Non-reactive in RPR assay. This antibody has not been absorbed and may react with related microorganisms.
  • Tested applicationsSuitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with Treponema pallidum. Not yet tested in other species.
  • Immunogen

    Tissue/ cell preparation (Highly purified T. pallidum).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 0.01M PBS, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notes>95% pure. The IgG fraction of the above antiserum covalently coupled with the N-Hydroxysuccinimide ester of biotin under mild conditions to give a high degree of substitution.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20465 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
ICC/IF
  • Application notesAvidin and streptavidin amplification systems for IF: Use at an assay dependent dilution.
    IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval using citrate buffer before commencing with IHC staining protocol.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceTreponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.
    • Alternative names
      • T. pallidum antibody

    References for Anti-Treponema pallidum antibody (Biotin) (ab20465)

    ab20465 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your enquiry. The IHC testing was done by a customer's laboratory. The antibody was used on paraffin sections after citrate antigen retrieval. They used an amplified colorimetric detection system, but I do not have any additional inf...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"