Purification notesPurified IgG fraction covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesIF: 1/10 - 1/50. Acetone fixation of the antigen source is recommended prior to staining.
IHC-P: Use at an assay dependent dilution.
Suitability for use in Western blot and ELISA has not been determined but use in such assays should not necessarily be excluded.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceTreponema pallidum is the causative agent of syphilis. It is a gram negative spirochete, a helical to sinusoidal bacterium with outer and cytoplasmic membranes, a thin peptidoglycan layer, and periplasmic flagella.
T. pallidum antibody
References for Anti-Treponema pallidum antibody (FITC) (ab20719)
has not yet been referenced specifically in any publications.
Publishing research using ab20719? Please let us know so that we can cite the reference in this datasheet.
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