Anti-TRF1 antibody [572C] (ab192629)
Key features and details
- Rat monoclonal [572C] to TRF1
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-TRF1 antibody [572C]
See all TRF1 primary antibodies -
Description
Rat monoclonal [572C] to TRF1 -
Host species
Rat -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant full length protein (His-tag) corresponding to Mouse TRF1 aa 1 to the C-terminus.
Database link: P70371-1 -
Positive control
- IHC: HEK-TRF1 and HEK-TRF2 transfected cell lines. WB: NIH-3T3 and Hek-TRF1 cell lysate, IPS-GFP-TRF1 homo cell lysate. ICC/IF: iPS cells,HRK-TRF1 and IPS-GFP-TRF1homo cell lines.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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Purity
Affinity purified -
Purification notes
HiTrap protein G columns -
Clonality
Monoclonal -
Clone number
572C -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa.
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 50 kDa. |
Target
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Function
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. -
Tissue specificity
Highly expressed and ubiquitous. Isoform Pin2 predominates. -
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain. -
Domain
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2. -
Post-translational
modificationsPhosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 7013 Human
- Entrez Gene: 21749 Mouse
- Omim: 600951 Human
- SwissProt: P54274 Human
- SwissProt: P70371 Mouse
- Unigene: 442707 Human
- Unigene: 4306 Mouse
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Alternative names
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
Images
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All lanes : Anti-TRF1 antibody [572C] (ab192629)
Lane 1 : IPS-GFP-TRF1 homo at 100 µg
Lane 2 : IPS-GFP-TRF1 heter at 100 µg
Lane 3 : IPS-sh TRF1 at 100 µg
Lane 4 : IPS-sh TRF1 puro at 100 µg
Lane 5 : HEK-TRF1 at 20 µg
Lane 6 : HEK-FGFR2 at 20 µg
Lane 7 : NIH-3T3 at 100 µg
Predicted band size: 50 kDaab192629 used undiluted for all lanes.
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Immunocytochemistry/ Immunofluorescent analysis of HEK-TRF1, HEK-GFP-TRF2, IPS-GFP-TRF1homo and IPS-shTRF1homo cell lines labeling TRF1 with undiluted ab192629.
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Immunocytochemistry/ Immunofluorescent analysis of frozen cytospins preparations of mTRF1 expressed in HEK293T labeling TRF1 using ab192629. Negative control on right used mTRF2-GFP expressed in HEK293T.
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All lanes : Anti-TRF1 antibody [572C] (ab192629) at 1/500 dilution
Lane 1 : Mefs TRF1 +/+ nuclear extract
Lane 2 : Mefs TRF1 KO nuclear extract
Lysates/proteins at 30 µg per lane.
Predicted band size: 50 kDa -
Immunocytochemistry/ Immunofluorescent analysis of mouse blastocyte labeling TRF1 with ab192629 at a 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab192629 has been referenced in 1 publication.
- Wang T et al. MFN2 Deficiency Impairs Mitochondrial Functions and PPAR Pathway During Spermatogenesis and Meiosis in Mice. Front Cell Dev Biol 10:862506 (2022). PubMed: 35493072