Anti-TRF1 antibody [3H11] (ab14397)

Overview

  • Product nameAnti-TRF1 antibody [3H11]
    See all TRF1 primary antibodies
  • Description
    Mouse monoclonal [3H11] to TRF1
  • Tested applicationsSuitable for: WB, ICC/IF, ELISpotmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human TRF1 protein.

  • Positive control
    • HeLa cell nuclear lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number3H11
  • MyelomaSp2/0-Ag14
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab14397 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100 - 1/200. Predicted molecular weight: 50 kDa.
ICC/IF Use a concentration of 1 µg/ml.
ELISpot Use 1µg for 106 cells.

Target

  • FunctionBinds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificityHighly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    modifications
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all

Anti-TRF1 antibody [3H11] images

  • ICC/IF image of ab14397 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab14397, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Anti-TRF1 antibody [3H11] (ab14397) at 1/200 dilution + HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing HeLa cells stained with ab14397 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14397, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TRF1 antibody [3H11] (ab14397)

ab14397 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (normal human fibroblasts)
Loading amount 60 µg
Specification normal human fibroblasts
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

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Verified customer

Submitted May 08 2006

Thank you for your enquiry. I am sorry to hear that you have been having difficulties with mouse monoclonal [3H11] to TRF1 (ab14397). I have reas teh details that you have submitted and I have a few comments. Please can you tell me whether you a...

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Thank you for your enquiry. The predicted molecular weight of human TRF1 is approximately 50 kDa. There was a mistake on the online datasheet for ab10579 and I have updated it. Thank you for bringing this to our attention and please contact us agai...

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Thank you for your reply, I appreciate your opinion on this matter and in light of the further information you sent me I agree that if you detected TRF1 without a problem you should see TRF2 too. Would you like to try a replacement vial or prefer a ...

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Many thanks for taking the time to provide all your protocol information, it is very useful to understand your problem and I'm sorry to hear you are experiencing unsatisfactory results with ab14397. I think your protocol is very good and the fact t...

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