Anti-TRF1 antibody - ChIP Grade (ab1423)
- Datasheet
- Specific References (28)
- Protocols
Overview
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Product nameAnti-TRF1 antibody - ChIP Grade
See all TRF1 primary antibodies -
DescriptionRabbit polyclonal to TRF1 - ChIP Grade
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Host speciesRabbit
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Tested applicationsSuitable for: ChIP, WB, ICC/IF, IPmore details
Unsuitable for: IHC-P -
Species reactivityReacts with: Mouse, Human
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Immunogen
Human full length recombinant protein expressed in bacteria.
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Positive control
- HL60 WCE lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.1% Sodium Azide
Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2 -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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ChIP Related Products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab1423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ChIP | Use at an assay dependent dilution. PubMed: 1946086 | |
| WB | 1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended. | |
| ICC/IF | Use at an assay dependent dilution. PubMed: 20427319 | |
| IP | Use at an assay dependent dilution. |
Application notes
Is unsuitable for IHC-P.
Target
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FunctionBinds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
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Tissue specificityHighly expressed and ubiquitous. Isoform Pin2 predominates.
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Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
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DomainThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2. -
Post-translational
modificationsPhosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. -
Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
- Information by UniProt
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Database links
- Entrez Gene: 7013 Human
- Entrez Gene: 21749 Mouse
- Omim: 600951 Human
- SwissProt: P54274 Human
- SwissProt: P70371 Mouse
- Unigene: 442707 Human
- Unigene: 4306 Mouse
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Alternative names
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
Images
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Western blot - Anti-TRF1 antibody - ChIP Grade (ab1423)Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected. Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected. -
Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody - ChIP Grade (ab1423)Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.
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Immunocytochemistry/ Immunofluorescence - Anti-TRF1 antibody - ChIP Grade (ab1423)The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).
Protocols
References
This product has been referenced in:
- Margalef P et al. Stabilization of Reversed Replication Forks by Telomerase Drives Telomere Catastrophe. Cell 172:439-453.e14 (2018). Mouse . Read more (PubMed: 29290468) »
- Ling X et al. TERT regulates telomere-related senescence and apoptosis through DNA damage response in male germ cells exposed to BPDE in vitro and to B[a]P in vivo. Environ Pollut 235:836-849 (2018). Mouse . Read more (PubMed: 29353801) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"