Overview

  • Product name
    Anti-TRF1 antibody - ChIP Grade
    See all TRF1 primary antibodies
  • Description
    Rabbit polyclonal to TRF1 - ChIP Grade
  • Tested applications
    Suitable for: ChIP, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human full length recombinant protein expressed in bacteria.

  • Positive control
    • HL60 WCE lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab1423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 1946086
WB 1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
ICC/IF Use at an assay dependent dilution. PubMed: 20427319
IP Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for IHC-P.
  • Target

    • Function
      Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
    • Tissue specificity
      Highly expressed and ubiquitous. Isoform Pin2 predominates.
    • Sequence similarities
      Contains 1 HTH myb-type DNA-binding domain.
    • Domain
      The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
      The TRFH dimerization region mediates the interaction with TINF2.
    • Post-translational
      modifications
      Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
      ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
    • Information by UniProt
    • Database links
    • Alternative names
      • hTRF1 AS antibody
      • NIMA interacting protein 2 antibody
      • NIMA-interacting protein 2 antibody
      • PIN 2 antibody
      • PIN2 antibody
      • t TRF1 antibody
      • Telomeric protein Pin2 antibody
      • Telomeric protein Pin2/TRF1 antibody
      • Telomeric repeat binding factor (NIMA interacting) 1 antibody
      • Telomeric repeat binding factor 1 antibody
      • Telomeric repeat binding protein 1 antibody
      • Telomeric repeat-binding factor 1 antibody
      • TERF 1 antibody
      • Terf1 antibody
      • TERF1_HUMAN antibody
      • TRBF 1 antibody
      • TRBF1 antibody
      • TRF 1 antibody
      • TRF antibody
      • TTAGGG repeat binding factor 1 antibody
      • TTAGGG repeat-binding factor 1 antibody
      see all

    Images

    • Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.
    • Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.

    • The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

    References

    This product has been referenced in:
    • Charif R  et al. Association of a Platinum Complex to a G-Quadruplex Ligand Enhances Telomere Disruption. Chem Res Toxicol N/A:N/A (2017). ChIP ; Human . Read more (PubMed: 28657713) »
    • Wang M  et al. Curcusone C induces telomeric DNA-damage response in cancer cells through inhibition of telomeric repeat factor 2. Biochim Biophys Acta 1865:1372-1382 (2017). ChIP ; Human . Read more (PubMed: 28870734) »

    See all 25 Publications for this product

    Customer reviews and Q&As

    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing (8% resolution gel)
    Sample
    Human Cell lysate - whole cell (hepatocellular carcinoma)
    Specification
    hepatocellular carcinoma
    Treatment
    Inhibition of one gene's expression using lentiviral shRNA
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 16 2013

    Thank you for your reply.

    Our datasheet for this antibody does not suggest any specific ICC protocol, as a general ICC protocol should work with this antibody. The protocol stated on the datasheet belongs to a customer’s review who subm...

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    Thank you for contacting us and bringing this to our attention. The switch to PFA fixation and detergent permeabilization instead of methanol fixation that I mentioned in our conversation may help, as that is what has worked for customers in the past. ...

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    Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Breast carcinoma)
    Specification
    Breast carcinoma
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X-100
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
    Username

    Abcam user community

    Verified customer

    Submitted Aug 23 2009

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HeLa cell)
    Loading amount
    100 µg
    Specification
    HeLa cell
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5%
    Username

    Dr. Geun Hyoung Ha

    Verified customer

    Submitted May 27 2009

    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela CELLS)
    Loading amount
    50 µg
    Specification
    Hela CELLS
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Mr. Geun-Hyoung Ha

    Verified customer

    Submitted Apr 30 2009

    Thank you for this information, it is very useful and I can now really understand the protocol and the optimisation steps. I think the WB protocol looks very good (apart from my suggestion of running a high percentage gel), the IP protocol we sugge...

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    I have carefully looked at the protocol and unfortunately need more information to understand the problem, I am sorry for the delay. The antibody has not been tested on HeLa cells, it does cross react with human but it is known that cancer cells ca...

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    I'm sorry to hear that this antibody is not working out for you. If you send me the Abcam order number or purchase order number that was used for your order for ab1423, I can give you a refund. There is no need to return the product to us.

    1-10 of 16 Abreviews or Q&A

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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