Customer reviews and FAQs

Overview

  • Product name
    Anti-TRF1 antibody - ChIP Grade
    See all TRF1 primary antibodies
  • Description
    Rabbit polyclonal to TRF1 - ChIP Grade
  • Specificity
    This antibody recognises TRF1. This antibody has not been tested against TFR2.
  • Tested applications
    Suitable for: ChIP, WB, ICC/IF, IPmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Human full length recombinant protein expressed in bacteria.

  • Positive control
    • HL60 WCE lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab1423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ChIP Use at an assay dependent dilution. PubMed: 1946086
WB 1/2000. Detects a band of approximately 60-65 kDa. An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.
ICC/IF Use at an assay dependent dilution. PubMed: 20427319
IP Use at an assay dependent dilution.
  • Application notes
    Is unsuitable for IHC-P.

    • Target

    • Function
      Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
    • Tissue specificity
      Highly expressed and ubiquitous. Isoform Pin2 predominates.
    • Sequence similarities
      Contains 1 HTH myb-type DNA-binding domain.
    • Domain
      The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
      The TRFH dimerization region mediates the interaction with TINF2.
    • Post-translational
      modifications
      Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
      ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
      Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
    • Cellular localization
      Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
    • Information by UniProt
    • Database links
      • Alternative names
        • hTRF1 AS antibody
        • NIMA interacting protein 2 antibody
        • NIMA-interacting protein 2 antibody
        • PIN 2 antibody
        • PIN2 antibody
        • t TRF1 antibody
        • Telomeric protein Pin2 antibody
        • Telomeric protein Pin2/TRF1 antibody
        • Telomeric repeat binding factor (NIMA interacting) 1 antibody
        • Telomeric repeat binding factor 1 antibody
        • Telomeric repeat binding protein 1 antibody
        • Telomeric repeat-binding factor 1 antibody
        • TERF 1 antibody
        • Terf1 antibody
        • TERF1_HUMAN antibody
        • TRBF 1 antibody
        • TRBF1 antibody
        • TRF 1 antibody
        • TRF antibody
        • TTAGGG repeat binding factor 1 antibody
        • TTAGGG repeat-binding factor 1 antibody
        see all

      Anti-TRF1 antibody - ChIP Grade images

      • Western blot - TRF1 antibody (ab1423)
        Western blot - TRF1 antibody (ab1423)
        Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.
      • Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)
        Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)

        Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.

      • Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)
        Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)
        The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).

      References for Anti-TRF1 antibody - ChIP Grade (ab1423)

      This product has been referenced in:
      • Charif R  et al. Association of a Platinum Complex to a G-Quadruplex Ligand Enhances Telomere Disruption. Chem Res Toxicol N/A:N/A (2017). ChIP ; Human . Read more (PubMed: 28657713) »
      • Su CH  et al. An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity. PLoS One 11:e0156378 (2016). ChIP ; Human . Read more (PubMed: 27228173) »

      See all 21 Publications for this product

      Publishing research using ab1423? Please let us know so that we can cite the reference in this datasheet.

      Product Wall

      Submit an abreview for ab1423 Submit a question for ab1423

      16 Abreviews or Q&A

      10
      Votes: Highest First

      Western blot abreview for Anti-TRF1 antibody - ChIP Grade

       Poor
      Abreviews
      Application
      Western blot
      Loading amount
      50 µg
      Gel Running Conditions
      Reduced Denaturing (8% resolution gel)
      Sample
      Human Cell lysate - whole cell (hepatocellular carcinoma)
      Specification
      hepatocellular carcinoma
      Treatment
      Inhibition of one gene's expression using lentiviral shRNA
      Blocking step
      Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Jul 16 2013

      Hi
      I’m surprise that this protocol is not the same as the one done by the technical sheet
      Indeed I did as recommended:
      - fixation with PFA 3.7% in PBS 5min

      - permeabilization with 0.5% triton-PBS for 5min

      - bl...

      Read More

      Thank you for your reply.

      Our datasheet for this antibody does not suggest any specific ICC protocol, as a general ICC protocol should work with this antibody. The protocol stated on the datasheet belongs to a customer’s review who subm...

      Read More

      The staining of TRF1 in MCF7 and another cell line is diffuse, and not primarily nuclear.

      Thank you for contacting us and bringing this to our attention. The switch to PFA fixation and detergent permeabilization instead of methanol fixation that I mentioned in our conversation may help, as that is what has worked for customers in the past. ...

      Read More

      I see a strong band at 50 kDa but only a very weak band at 60-65 kDa, where the antibody datasheet says this is observed. Other TRF1 antibody datasheets also show the protein at 60 kDa.

      Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

      I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

      Read More

      Immunocytochemistry/ Immunofluorescence abreview for Anti-TRF1 antibody - ChIP Grade

       Average
      Abreviews
      Abcam guarantees this product to work in the species/application used in this Abreview.
      Application
      Immunocytochemistry/ Immunofluorescence
      Sample
      Human Cell (Breast carcinoma)
      Specification
      Breast carcinoma
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.5% Triton X-100
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 37°C
      Read More
      abreview image
      Username

      Abcam user community

      Verified customer

      Submitted Aug 23 2009

      Western blot abreview for Anti-TRF1 antibody - ChIP Grade

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HeLa cell)
      Loading amount
      100 µg
      Specification
      HeLa cell
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5%
      Read More
      abreview image
      Username

      Dr. Geun Hyoung Ha

      Verified customer

      Submitted May 27 2009

      Western blot abreview for Anti-TRF1 antibody - ChIP Grade

       Poor
      Abreviews
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (Hela CELLS)
      Loading amount
      50 µg
      Specification
      Hela CELLS
      Gel Running Conditions
      Reduced Denaturing (8%)
      Blocking step
      Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More
      Username

      Mr. Geun-Hyoung Ha

      Verified customer

      Submitted Apr 30 2009

      follow up to previous correspondance: 1) Did the customer aliquot the vial upon receipt and uses a fresh aliquot every time? -> yes!! 2) Is the HeLa cell extract a nuclear extract? The protein is a nuclear protein and absence in the WB co...

      Read More

      Thank you for this information, it is very useful and I can now really understand the protocol and the optimisation steps. I think the WB protocol looks very good (apart from my suggestion of running a high percentage gel), the IP protocol we sugge...

      Read More

      Our end user claimed about ab1423. She performed western blot , and IP, but she coull not detect band. When she performed westernblot, she expected 60-65 KDa band according your data sheet. But she could detect 75KDa band. Please confirm this....

      Read More

      I have carefully looked at the protocol and unfortunately need more information to understand the problem, I am sorry for the delay. The antibody has not been tested on HeLa cells, it does cross react with human but it is known that cancer cells ca...

      Read More

      Hi, Thank you for your time and for all the information. My protocol is almost identical to this one that you gave me. I also tried two other protocols. If it looked like I was getting something close to what I expect then I would persevere but...

      Read More

      I'm sorry to hear that this antibody is not working out for you. If you send me the Abcam order number or purchase order number that was used for your order for ab1423, I can give you a refund. There is no need to return the product to us.

      1-10 of 16 Abreviews or Q&A

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