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Products:Epigenetics and Nuclear Signaling >> Chromosome Structure >> Telomeres
Overview
Product name
Anti-TRF1 antibody - ChIP Grade
See all TRF1 products (15) ...
Description
Rabbit polyclonal to TRF1 - ChIP Grade
Specificity
This antibody recognises TRF1. This antibody has not been tested against TFR2.
Tested applications
ChIP, WB, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Human full length recombinant protein expressed in bacteria.
Positive control
HL60 WCE lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 0.1M Sodium chloride, 0.01M PBS, pH 7.2
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab1423 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 1946086)
WB: 1/2000Detects a band of approximately 60-65 kDa.(An additional non specific band at 35 kDa may be observed. Denatured whole cell extracts (prepare total cell extracts with 6M urea, 1% SDS, 150 mM NaCl, 25mM Tris ph8, and then sonicate for 10 seconds)and TBS based buffers with 0.5% Tween 20 are recommended.)
ICC/IF: Use at an assay dependent dilution. (PubMed: 20427319)
IP: Use at an assay dependent dilution.
Application notes
Is unsuitable for or IHC-P.
Target
Function
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
Tissue specificity
Highly expressed and ubiquitous. Isoform Pin2 predominates.
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain.
Domain
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2.
Post-translational
modifications
Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
Target information above from: UniProt accessionP54274
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
Database links
- Entrez Gene: 7013 Human
- Entrez Gene: 21749 Mouse
- Omim: 600951 Human
- SwissProt: P54274 Human
- SwissProt: P70371 Mouse
- Unigene: 442707 Human
- Unigene: 4306 Mouse
Anti-TRF1 antibody - ChIP Grade images:
Western blot - TRF1 antibody (ab1423)
Western blot using anti-TRF1 at 1/2000. A single band at 60-65 kD is detected.
Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)
Cells transfected with a TRF1-GFP contruct. On the left: immunofluorescence with the anti-TRF1 antibody used at 1/300, with a Texas Red-conjugated secondary antibody. In the centre: fluorescence from the GFP. Right: merge of both images showing overlap of GFP fluorescence and antibody staining.
Immunocytochemistry/ Immunofluorescence - TRF1 antibody (ab1423)
The cells in the first image are probably in a stage of the cell cycle where the signal is not so clear. In this image the antibody is shown to recognise the same signal but staining is shown as 20-30 spots (punctate foci).
References for Anti-TRF1 antibody - ChIP Grade (ab1423)
This product has been referenced in:
- Choi KHet al. Characterization of the DNA binding specificity of Shelterin complexes. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21852327) »
- Fakhoury Jet al. Telomeric function of mammalian telomerases at short telomeres. J Cell Sci 123:1693-704 (2010). ICC/IF; Human.Read more (PubMed: 20427319) »
See all 9 publications for this product
Publishing research using ab1423? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"