Recombinant Anti-TRF1 antibody [EPR6313] (ab129177)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6313] to TRF1
- Suitable for: WB
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-TRF1 antibody [EPR6313]
See all TRF1 primary antibodies -
Description
Rabbit monoclonal [EPR6313] to TRF1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: K562 cell lysate, 293T cell lysate, Caco 2 cell lysate, HeLa whole cell lysate (ab150035)., HEK-293 cell lysate, Mouse 14 day embryo lysates;
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6313 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab129177 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 50 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Binds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. -
Tissue specificity
Highly expressed and ubiquitous. Isoform Pin2 predominates. -
Sequence similarities
Contains 1 HTH myb-type DNA-binding domain. -
Domain
The acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
The TRFH dimerization region mediates the interaction with TINF2. -
Post-translational
modificationsPhosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle. - Information by UniProt
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Database links
- Entrez Gene: 7013 Human
- Entrez Gene: 21749 Mouse
- Omim: 600951 Human
- SwissProt: P54274 Human
- SwissProt: P70371 Mouse
- Unigene: 442707 Human
- Unigene: 4306 Mouse
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Alternative names
- hTRF1 AS antibody
- NIMA interacting protein 2 antibody
- NIMA-interacting protein 2 antibody
see all
Images
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Anti-TRF1 antibody [EPR6313] (ab129177) at 1/1000 dilution (Purified) + Mouse 14 day embryo lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Anti-TRF1 antibody [EPR6313] (ab129177) at 1/1000 dilution (Purified) + HEK-293 (Human embryonic kidney epithelial cell) whole cell lysates at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
All lanes : Anti-TRF1 antibody [EPR6313] (ab129177) at 1/1000 dilution (Unpurified)
Lane 1 : K562 (human chronic myelogenous leukemia cell line from bone marrow) cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 3 : Caco-2 (Human colorectal adenocarcinoma cell line) cell lysate
Lane 4 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution
Predicted band size: 50 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab129177 has been referenced in 4 publications.
- Kychygina A et al. Progerin impairs 3D genome organization and induces fragile telomeres by limiting the dNTP pools. Sci Rep 11:13195 (2021). PubMed: 34162976
- Yang L et al. TAZ maintains telomere length in TNBC cells by mediating Rad51C expression. Breast Cancer Res 23:89 (2021). PubMed: 34488828
- Fang MY et al. Small-Molecule Modulation of TDP-43 Recruitment to Stress Granules Prevents Persistent TDP-43 Accumulation in ALS/FTD. Neuron 103:802-819.e11 (2019). PubMed: 31272829
- Episkopou H et al. TSPYL5 Depletion Induces Specific Death of ALT Cells through USP7-Dependent Proteasomal Degradation of POT1. Mol Cell 75:469-482.e6 (2019). PubMed: 31278054