Anti-TRF1 (phospho S219) antibody (ab79187)

Overview

  • Product nameAnti-TRF1 (phospho S219) antibody
    See all TRF1 primary antibodies
  • Description
    Rabbit polyclonal to TRF1 (phospho S219)
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized phosphopeptide derived from human TRF1 around the phosphorylation site of serine 219 (I-I-SP-Q-K).

  • Positive control
    • 293 lysate treated with paclitaxel (1 µM, 24 hours)

Properties

  • FormLiquid
  • Storage instructionsFrozen Stock (-20C). Shelf life 12 months.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAffinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab79187 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 50 kDa.
ELISA 1/40000.

Target

  • FunctionBinds the telomeric double-stranded TTAGGG repeat and negatively regulates telomere length. Involved in the regulation of the mitotic spindle. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways.
  • Tissue specificityHighly expressed and ubiquitous. Isoform Pin2 predominates.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe acidic N-terminal domain binds to the ankyrin repeats of TNKS1 and TNKS2. The C-terminal domain binds microtubules.
    The TRFH dimerization region mediates the interaction with TINF2.
  • Post-translational
    modifications
    Phosphorylated preferentially on Ser-219 in an ATM-dependent manner in response to ionizing DNA damage.
    ADP-ribosylation by TNKS1 or TNKS2 diminishes its ability to bind to telomeric DNA.
    Ubiquitinated by RLIM/RNF12, leading to its degradation by the proteasome. Ubiquitinated by a SCF (SKP1-CUL1-F-box protein) ubiquitin-protein ligase complex, leading to its degradation by the proteasome.
  • Cellular localizationNucleus. Cytoplasm > cytoskeleton > spindle. Chromosome > telomere. Colocalizes with telomeric DNA in interphase and metaphase cells and is located at chromosome ends during metaphase. Associates with the mitotic spindle.
  • Information by UniProt
  • Database links
  • Alternative names
    • hTRF1 AS antibody
    • NIMA interacting protein 2 antibody
    • NIMA-interacting protein 2 antibody
    • PIN 2 antibody
    • PIN2 antibody
    • t TRF1 antibody
    • Telomeric protein Pin2 antibody
    • Telomeric protein Pin2/TRF1 antibody
    • Telomeric repeat binding factor (NIMA interacting) 1 antibody
    • Telomeric repeat binding factor 1 antibody
    • Telomeric repeat binding protein 1 antibody
    • Telomeric repeat-binding factor 1 antibody
    • TERF 1 antibody
    • Terf1 antibody
    • TERF1_HUMAN antibody
    • TRBF 1 antibody
    • TRBF1 antibody
    • TRF 1 antibody
    • TRF antibody
    • TTAGGG repeat binding factor 1 antibody
    • TTAGGG repeat-binding factor 1 antibody
    see all

Anti-TRF1 (phospho S219) antibody images

  • Lanes 1 - 2 : Anti-TRF1 (phospho S219) antibody (ab79187) at 1/500 dilution
    Lane 3 : A different TRF1 antibody (ab63374)

    Lane 1 : 293 cells treated with paclitaxel (1 µM, 24 hours)
    Lane 2 : 293 cells treated with paclitaxel (1 µM, 24 hours) with immunizing phosphopeptide at 10 µg
    Lane 3 : 293 cells treated with paclitaxel (1 µM, 24 hours)

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 50 kDa
    Observed band size : 50 kDa

References for Anti-TRF1 (phospho S219) antibody (ab79187)

ab79187 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"