Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)


  • Product nameAnti-TRF2 antibody [4A794] - ChIP Grade
    See all TRF2 primary antibodies
  • Description
    Mouse monoclonal [4A794] to TRF2 - ChIP Grade
  • Tested applicationsSuitable for: IHC-P, WB, ICC, IP, ChIP, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Indian Muntjac
  • Immunogen

    Baculovirus expressing full length TRF2 protein.

  • Positive control
    • Jurkat cell lysate (WB)



Our Abpromise guarantee covers the use of ab13579 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 1 µg/ml.
WB Use a concentration of 4 µg/ml. Detects a band of approximately 66 kDa.
ICC Use at an assay dependent concentration.
IP Use 2µg for 106 cells.
ChIP Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration. PubMed: 20679250
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.


  • FunctionBinds the telomeric double-stranded 5'-TTAGGG-3' repeat and plays a central role in telomere maintenance and protection against end-to-end fusion of chromosomes. In addition to its telomeric DNA-binding role, required to recruit a number of factors and enzymes required for telomere protection, including the shelterin complex, TERF2IP/RAP1 and DCLRE1B/Apollo. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded 5'-TTAGGG-3' repeats added by telomerase and protects chromosome ends; without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Together with DCLRE1B/Apollo, plays a key role in telomeric loop (T loop) formation by generating 3' single-stranded overhang at the leading end telomeres: T loops have been proposed to protect chromosome ends from degradation and repair. Required both to recruit DCLRE1B/Apollo to telomeres and activate the exonuclease activity of DCLRE1B/Apollo. Preferentially binds to positive supercoiled DNA. Together with DCLRE1B/Apollo, required to control the amount of DNA topoisomerase (TOP1, TOP2A and TOP2B) needed for telomere replication during fork passage and prevent aberrant telomere topology. Recruits TERF2IP/RAP1 to telomeres, thereby participating in to repressing homology-directed repair (HDR), which can affect telomere length.
  • Tissue specificityUbiquitous. Highly expressed in spleen, thymus, prostate, uterus, testis, small intestine, colon and peripheral blood leukocytes.
  • Sequence similaritiesContains 1 HTH myb-type DNA-binding domain.
  • DomainThe TRFH dimerization region mediates the interaction with DCLRE1B/Apollo but not TINF2.
  • Post-translational
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. Chromosome > telomere. Colocalizes with telomeric DNA in interphase cells and is located at chromosome ends during metaphase.
  • Information by UniProt
  • Database links
  • Alternative names
    • Telomeric DNA binding protein antibody
    • Telomeric DNA-binding protein antibody
    • Telomeric repeat binding factor 2 antibody
    • Telomeric repeat binding protein 2 antibody
    • Telomeric repeat-binding factor 2 antibody
    • TERF 2 antibody
    • Terf2 antibody
    • TERF2_HUMAN antibody
    • TRBF 2 antibody
    • TRBF2 antibody
    • TRF 2 antibody
    • TTAGGG repeat binding factor 2 antibody
    • TTAGGG repeat-binding factor 2 antibody
    see all

Anti-TRF2 antibody [4A794] - ChIP Grade images

  • ab13579 staining TRF2 in bladder carcinoma cells using Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Antigeen retrieval carried out by heat treatment in Citrate buffer pH6.0. Primary antibody diluted to 4μg/ml.

  • Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579.  2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.

    Immunoprecipitation / Western Blot analysis of TRF2 in HL60 cells. 1. IP with ab13579. 2. IP with control mouse IgG. TRF2 is detected as an ~ 66 kD protein.
  • ab13579 (1µg/ml) staining TRF2 in human spleen, using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining in the germinal follicle.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ab13579 staining TRF2 in HeLa cells by Immunocytochemistry/ Immunofluorescence.
    The cells were washed in PBS, fixed for 15 minutes at room temperature in 4% formaldehyde in PBS, and then washed for 5 minutes with PBS. The cells were then dehydrated with cold methanol at room temperature for 10 minutes, washed, and blocked with blocking solution (3% skim milk, 0.05% Tween-20 in PBS) at room temperature for 1 hour. ab13579 added at 2 µg/ml at 37?°C for 1 hour. After washing three times, 5 µg/mL fluorescein (TRITC) goat anti-mouse (Abcam) as the secondary antibody in blocking solution was added and incubated at room temperature for 1 hour.
  • Overlay histogram showing HeLA cells stained with ab13579 (red line). The cells were fixed with 100% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab13579, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was Mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-TRF2 antibody [4A794] - ChIP Grade (ab13579)

This product has been referenced in:
  • Su CH  et al. An H2A Histone Isotype, H2ac, Associates with Telomere and Maintains Telomere Integrity. PLoS One 11:e0156378 (2016). ChIP ; Human . Read more (PubMed: 27228173) »
  • Yuan P  et al. Telomerase Cajal body protein 1 depletion inhibits telomerase trafficking to telomeres and induces G1 cell cycle arrest in A549 cells. Oncol Lett 8:1009-1016 (2014). ICC/IF ; Human . Read more (PubMed: 25120649) »

See all 12 Publications for this product

Product Wall

I have heard back from the lab regarding the antibodies ab13579, ab14402 & ab111118:

Ab13579 : Full-length recombinant human TRF2 protein was used as immunogen to generate the antibody.

We have not mapped the epitope...

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Thank you for your reply.

We would be thankfull if you could fill out the attached questionnaire, so we can get the key facts about your protocol to further analyze this failure.

As requested, I have issued a free of charge re...

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Selon nos informations vous avez connu quelques difficultés avec ab13579 et avez contacté notre service scientifique.

Nous vous avions donné des suggéstions pour améliorer les résultats. Je vous &e...

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Thank you for your enquiry.

ab96876 is a Donkey polyclonal anti mouse IgG secondary, and should therefore detect ab13579TRF2 antibody [4A794]which is a mouse IgG1 primary.

As stated on the datasheet, ab96876 has been tested in IHC...

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Merci d'avoir pris le temps de remplir notre questionnaire et de nous avoir contactés. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ab13579.

Comme déjà expliqué, notre politique est d'offrir tout d'abord ...

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Je suis désolée d'apprendre que ab13579 ne vous donne pas de bons résultats en immunohistochimie. Nous n'avons pas eu des autres retours négatives sur ce lot ou sur cet anticorps. J'aimerai bien investiguer le problème que vous avez rencontré un peu pl...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat cells)
Loading amount 200000 cells
Specification Jurkat cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Nov 25 2009

Thank you for your enquiry and your patience. There is no explanation for a band only observed at 70 kDa and we have not observed this band previously. I contacted the originator of the antibody and they have also not observed this band. Could you...

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I'm sorry to hear you are having a problem with ab10579. Could you please fill out the form at the following link as we discussed on the phone? ...

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